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A defined locus control region determinant links chromatin domain acetylation with long-range gene activation
Journal article   Open access   Peer reviewed

A defined locus control region determinant links chromatin domain acetylation with long-range gene activation

Yugong Ho, Felice Elefant, Nancy Cooke and Stephen Liebhaber
Molecular cell, v 9(2), pp 291-302
Feb 2002
PMID: 11864603
url
https://doi.org/10.1016/S1097-2765(02)00447-1View
Published, Version of Record (VoR) Open

Abstract

Chromatin - metabolism Sequence Deletion Humans Transcriptional Activation Transcription Factor Pit-1 Pituitary Gland, Anterior - metabolism DNA-Binding Proteins - metabolism Female Transcription, Genetic Acetylation Locus Control Region - physiology Transgenes Binding Sites Promoter Regions, Genetic Trophoblasts - metabolism Gene Expression Regulation - genetics Human Growth Hormone - genetics Mice, Transgenic Organ Specificity Pregnancy Transcription Factors - metabolism Animals Mice Protein Processing, Post-Translational Histones - metabolism Chromatin - genetics
Gene activation in higher eukaryotes is often under the control of regulatory elements quite distant from their target promoters. It is unclear how such long-range control is mediated. Here we show that a single determinant of the human growth hormone locus control region (hGH LCR) located 14.5 kb 5prime prime or minute to the hGH-N promoter has a critical, specific, and nonredundant role in facilitating promoter trans factor binding and activating hGH-N transcription. Significantly, this same determinant plays an essential role in establishing a 32 kb acetylated domain that encompasses the entire hGH LCR and the contiguous hGH-N promoter. These data support a model for long-range gene activation via LCR-mediated targeting and extensive spreading of core histone acetylation.

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Web of Science research areas
Biochemistry & Molecular Biology
Cell Biology
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