Journal article
A dual-mode aptasensor based on rolling circle amplification enriched G-quadruplex for highly sensitive IFN-γ detection
Analytica chimica acta, v 1329, 343254
15 Nov 2024
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Abstract
Aptasensors have been extensively utilized in target detection due to their advantages of high sensitivity and fast response. However, the reliability of the detection results of the single-mode aptasensor cannot be verified in time. Developing efficient detection methods with cross-validation capability is beneficial to achieving highly reliable detection. This study aims to design a colorimetric and fluorescent dual-mode aptasensor by skillfully engineering G-quadruplex assembly and rolling circle amplification for highly reliable IFN-γ detection.
The complexes of anti–IFN–γ aptamers and complement sequences (cDNA) were modified on the magnetic beads. In the presence of IFN-γ, the preferential combination of aptamers with IFN-γ resulted in the release of cDNAs. The cDNAs were collected by magnetic separation and then used as primers to trigger rolling circle amplification reaction to generate enriched G-quadruplexes. The G-quadruplex could be utilized to combine with hemin to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine for colormetric mode or to couple with the fluorogenic dye Thioflavin T for fluorescent mode. The developed dual-mode aptasensor displayed a linear range of 1–10000 pM with a detection limit of 0.406 pM for the colormetric mode and a range of 0.1–10000 pM with a detection limit of 0.037 pM for the fluorescent mode. Further, the designed aptasensor was applied to IFN-γ detection in serum samples and achieved satisfactory recoveries.
This innovative dual-mode detection strategy skillfully leverages the effective target-binding ability of aptamer, dual-function of the G-quadruplex and the signal amplifying ability of rolling circle amplification. This approach not only provides a reliable testing tool for the detection of IFN-γ, but also promotes the development of multimode sensing platforms.
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•A novel colorimetric and fluorescent dual-mode aptasensor was successfully constructed.•RCA-enriched G-quadruplex was skillfully utilized in the dual-mode aptasensor.•The analytical performances of aptasensor were validated to be sensitive, specific and accurate.
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Details
- Title
- A dual-mode aptasensor based on rolling circle amplification enriched G-quadruplex for highly sensitive IFN-γ detection
- Creators
- Lianhui Zhao - Beijing University of Chemical TechnologyYingai Yin - Beijing University of Chemical TechnologyShuqi Xiao - Beijing University of Chemical TechnologyYinghua Qiu - Drexel UniversitySai Wang - Ocean University of ChinaYiyang Dong - Beijing University of Chemical Technology
- Publication Details
- Analytica chimica acta, v 1329, 343254
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- Fundamental Research Funds the Central Universities: 202341012 Beijing versity of Chemical Technology-China-Japan - China-Japan Friendship Hospital Biomedical Transformation Engineering Research Center Joint: RZ2020-02
This research was supported by the Fundamental Research Funds the Central Universities (Grant No. 202341012) and the Beijing versity of Chemical Technology-China-Japan - China-Japan Friendship Hospital Biomedical Transformation Engineering Research Center Joint (Grant No. RZ2020-02) .
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001321317600001
- Scopus ID
- 2-s2.0-85204353114
- Other Identifier
- 991021904629104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical