Journal article
A gene highly expressed in tumor cells encodes novel structure proteins
Oncogene, v 23(58), pp 9438-9446
16 Dec 2004
PMID: 15602574
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We isolated several related but distinct cDNA clones encoding novel structure proteins (NSP) when screening a cDNA library. Analysis revealed that these cDNAs and several similar ESTs in the public databases are derived from a single gene of 17 exons that span a minimum of 227-kb region. This gene is located at chromosome 17p11.2, a region frequently amplified in human gliomas and osteosarcomas, and involved in Birt–Hogg–Dube syndrome, a tumor-prone syndrome. The major coding sequences shared by all isolated transcripts are predicted to encode SMC (structural maintenance of chromosome)/SbcC ATPase motifs and coiled-coil domains commonly seen in motor or structure proteins. Two 5′-end and two 3′-end variants (type 5
α/β
and 3
α/β
, respectively) were identified, making a total of four possible transcripts. Both 5
α
and 5
β
variants were detected in human testis mRNA, but only type 5
α
was detectable in RNA samples extracted from HeLa cells. The unique carboxyl-terminus of 3
β
contains a Ca
2+
-dependent actin-binding domain. Immunohistochemistry studies revealed that NSPs were mostly localized to nuclei. Northern blot analysis demonstrated two major bands and the expression levels are tremendously high in testis while barely detectable in other normal tissues examined. Interestingly, NSP5α3α is highly expressed in some tumor cell lines. These results suggest that NSPs represent a new family of structure proteins with a possible role in nuclear dynamics during cell division, and that
NSP5α
3
α
may serve as a tumor marker.
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Details
- Title
- A gene highly expressed in tumor cells encodes novel structure proteins
- Creators
- Nianli Sang - Program of Molecular Biology, Temple University, Philadelphia, PA 19122, USADonna M Fath - Cardeza Foundation for Hematological Research and Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USAAntonio Giordano - Program of Molecular Biology, Temple University, Philadelphia, PA 19122, USA
- Publication Details
- Oncogene, v 23(58), pp 9438-9446
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000225764100016
- Scopus ID
- 2-s2.0-11244308119
- Other Identifier
- 991014877782704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- Genetics & Heredity
- Oncology