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Accepted (AM)Open Access (License Unspecified), Open
Journal article
A high-efficiency superhydrophobic plasma separator
Lab on a chip, v 16(3), pp 553-560
07 Feb 2016
PMCID: PMC4729584
PMID: 26732765
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To meet stringent limit-of-detection specifications for low abundance target molecules, a relatively large volume of plasma is needed for many blood-based clinical diagnostics. Conventional centrifugation methods for plasma separation are not suitable for on-site testing or bedside diagnostics. Here, we report a simple, yet high-efficiency, clamshell-style, superhydrophobic plasma separator that is capable of separating a relatively large volume of plasma from several hundred microliters of whole blood (finger-prick blood volume). The plasma separator consists of a superhydrophobic top cover with a separation membrane and a superhydrophobic bottom substrate. Unlike previously reported membrane-based plasma separators, the separation membrane in our device is positioned at the top of the sandwiched whole blood film to increase the membrane separation capacity and plasma yield. In addition, the device's superhydrophobic characteristics (i) facilitates the formation of well-defined, contracted, thin blood film with a high contact angle; (ii) minimizes biomolecular adhesion to surfaces; (iii) increases blood clotting time; and (iv) reduces blood cell hemolysis. The device demonstrated a "blood in-plasma out" capability, consistently extracting 65 +/- 21.5 mu L of plasma from200 mu L of whole blood in less than 10 min without electrical power. The device was used to separate plasma from Schistosoma mansoni genomic DNA-spiked whole blood with a recovery efficiency of > 84.5 +/- 25.8%. The S. mansoni genomic DNA in the separated plasma was successfully tested on our custom-made microfluidic chip by using loop mediated isothermal amplification (LAMP) method.
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Details
- Title
- A high-efficiency superhydrophobic plasma separator
- Creators
- Changchun Liu - University of PennsylvaniaShih-Chuan Liao - University of PennsylvaniaJinzhao Song - University of PennsylvaniaMichael G. Mauk - Univ Penn, Dept Mech Engn & Appl Mech, 210 Towne Bldg,220 South 33rd St, Philadelphia, PA 19104 USAXuanwen Li - Translational TherapeuticsGaoxiang Wu - Philadelphia UniversityDengteng Ge - Philadelphia UniversityRobert M. Greenberg - College Station Medical CenterShu Yang - Philadelphia UniversityHaim H. Bau - University of Pennsylvania
- Publication Details
- Lab on a chip, v 16(3), pp 553-560
- Publisher
- Royal Soc Chemistry
- Number of pages
- 8
- Grant note
- K25AI099160; R21AI112713; R41AI104418 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30AI045008 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) AI045008 / Penn CFAR Pilot Grant
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Leadership and Society/Engineering Technology
- Web of Science ID
- WOS:000368858700016
- Scopus ID
- 2-s2.0-84956763425
- Other Identifier
- 991020623757304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Multidisciplinary
- Instruments & Instrumentation
- Nanoscience & Nanotechnology