Journal article
A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris
Protein expression and purification, v 74(1), pp 9-15
01 Nov 2010
PMID: 20447459
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Glycoengineered Pichia pastoris provides a unique platform for screening monoclonal antibody (mAb) leads and high expressing strains. A simple, economic, and high-throughput purification for mAb from P. pastoris fermentation has been developed that can be easily operated in various commercially available liquid handlers. The method includes the use of STREAMLINE rProtein A in a 96-well platform and demonstrates good linear alignment and reproducibility in a wide concentration range. The antibody titers measured by the method have less than 15% variation in comparison to spiking titers. The mAb titer and quality obtained from this method are comparable to that from conventional column chromatography. The method can process hundreds of expression screening samples in a day, not only to accurately determine titers, but also to generate milligram quantities of mAb for quality assessment, including purity, folding, glycosylation, and antigen binding affinity. (C) 2010 Elsevier Inc. All rights reserved.
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Details
- Title
- A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris
- Creators
- Youwei Jiang - GlycoFi Inc, Lebanon, NH 03766 USAFang Li - GlycoFi Inc, Lebanon, NH 03766 USAMichelle Button - GlycoFi Inc, Lebanon, NH 03766 USAMichael Cukan - GlycoFi Inc, Lebanon, NH 03766 USARenee Moore - GlycoFi Inc, Lebanon, NH 03766 USANathan Sharkey - GlycoFi Inc, Lebanon, NH 03766 USAHuijuan Li - GlycoFi Inc, Lebanon, NH 03766 USA
- Publication Details
- Protein expression and purification, v 74(1), pp 9-15
- Publisher
- Elsevier
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Epidemiology and Biostatistics
- Web of Science ID
- WOS:000281495100002
- Scopus ID
- 2-s2.0-77957882415
- Other Identifier
- 991021448032104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology