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A highly conserved region upstream of the fibronectin alternative exon EIIIA 3′ splice site interacts with cell-type-specific nuclear proteins
Journal article   Peer reviewed

A highly conserved region upstream of the fibronectin alternative exon EIIIA 3′ splice site interacts with cell-type-specific nuclear proteins

Pamela A Norton, Tatyana Uporova and Vickie D Bennett
Biochimica et biophysica acta. Gene structure and expression, v 1395(2)
1998
PMID: 9473640

Abstract

Alternative splicing Chondrogenesis Exon EIIIA Fibronectin Regulatory element
Sequencing of chicken fibronectin genomic DNA and interspecies sequence comparisons reveal a highly conserved region upstream of the alternatively spliced exon EIIIA. UV-crosslinking of RNAs corresponding to this region from the chicken and rat genes with HeLa nuclear extract demonstrates that both RNAs interact with similar proteins. However, both RNAs crosslink to a 70 kDa protein present in nuclear extracts from cells and tissues that include exon EIIIA, but not in extracts from tissues that exclude the exon. This protein represents a candidate cell-type-specific factor involved in exon EIIIA inclusion.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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