Journal article
A large volume, portable, real-time PCR reactor
Lab on a chip, v 10(22), pp 3170-3177
21 Nov 2010
PMID: 20927453
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A point-of-care, diagnostic system incorporating a portable thermal cycler and a compact fluorescent detector for real-time, polymerase chain reaction (PCR) on disposable, plastic microfluidic reactors with relatively large reaction volume (ranging from 10 µL to 100 µL) is described. To maintain temperature uniformity and a relatively fast temperature ramping rate, the system utilizes double-sided heater that features a master, thermoelectric element and a thermal waveguide connected to a second thermoelectric element. The waveguide has an aperture for optical coupling between a miniature, fluorescent reader and the PCR reaction chamber. The temperature control is accomplished with a modified, feedforward, variable structural proportional-integral-derivative controller. The temperature of the liquid in the reaction chamber tracks the set-point temperature with an accuracy of ± 0.1 °C. The transition times from one temperature to another are minimized with controllable overshoots (< 2 °C) and undershoots (< 5 °C). The disposable, single-use PCR chip can be quickly inserted into a thermal cycler/reader unit for point-of-care diagnostics applications. The large reaction chamber allows convenient pre-storing of dried, paraffin-encapsulated PCR reagents (polymerase, primers, dNTPs, dyes, and buffers) in the PCR chamber. The reagents are reconstituted "just in time" by heating during the PCR process. The system was tested with viral and bacterial nucleic acid targets.
Metrics
Details
- Title
- A large volume, portable, real-time PCR reactor
- Creators
- Xianbo Qiu - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USAMichael G MaukDafeng ChenChangchun LiuHaim H Bau
- Publication Details
- Lab on a chip, v 10(22), pp 3170-3177
- Publisher
- Royal Society of Chemistry; England
- Grant note
- U01DE017855 / NIDCR NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000283600900019
- Scopus ID
- 2-s2.0-78049260089
- Other Identifier
- 991014878225804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
Source: SDGs in the Output
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Multidisciplinary
- Instruments & Instrumentation
- Nanoscience & Nanotechnology