Journal article
A mechanism for heterogeneous endothelial responses to flow in vivo and in vitro
Journal of biomechanics, v 28(12), pp 1553-1560
1995
PMID: 8666594
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Exposure of endothelium to a nominally uniform flow field
in vivo and
in vitro frequently results in a heterogeneous distribution of individual cell responses. Extremes in response levels are often noted in neighboring cells. Such variations are important for the spatial interpretation of vascular responses to flow and for an understanding of mechanotransduction mechanisms at the level of single cells. We propose that variations of local forces defined by the cell surface geometry contribute to these differences. Atomic force microscopy measurements of cell surface topography in living endothelium both
in vitro and
in situ combined with computational fluid dynamics demonstrated large cell-to-cell variations in the distribution of flow-generated shear stresses at the endothelial luminal surface. The distribution of forces throughout the surface of individual cells of the monolayer was also found to vary considerably and to be defined by the surface geometry. We conclude that the endothelial three-dimensional surface geometry defines the detailed distribution of shear stresses and gradients at the single cell level, and that there are large variations in force magnitude and distribution between neighboring cells. The measurements support a topographic basis for differential endothelial responses to flow observed
in vivo and
in vitro. Included in these studies are the first preliminary measurements of the living endothelial cell surface in an intact artery.
Metrics
Details
- Title
- A mechanism for heterogeneous endothelial responses to flow in vivo and in vitro
- Creators
- Peter F Davies - Department of Pathology, The University of Chicago, 5841 S. Maryland Avenue, Chicago, IL 60637, U.S.ATrevor Mundel - Department of Mathematics, The University of Chicago, 5841 S. Maryland Avenue, Chicago, IL 60637, U.S.AKenneth A Barbee - Department of Pathology, The University of Chicago, 5841 S. Maryland Avenue, Chicago, IL 60637, U.S.A
- Publication Details
- Journal of biomechanics, v 28(12), pp 1553-1560
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:A1995TL56400014
- Scopus ID
- 2-s2.0-0029583932
- Other Identifier
- 991014877651404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Engineering, Biomedical