Journal article
A methacrylated hyaluronic acid network reinforced Pluronic F-127 gel for treatment of bacterial keratitis
Biomedical materials (Bristol), v 17(4), p45017
11 May 2022
PMID: 35545060
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this study, we developed a novel in situ thermoresponsive gel by introducing crosslinked methacrylated hyaluronic acid (HA-MA) networks into Pluronic F-127 (PF-127) gel (HP gel) to achieve levofloxacin (LFX) delivery in bacterial keratitis treatment. The interactions between PF-127 and HA-MA networks were studied by scanning electron microscopy, rheology, dynamic light scattering, differential scanning calorimetry, and small angle X-ray scattering. The results showed that the HP gel exhibited a higher critical gelling temperature and lower viscosity than the PF-127 gel (P gel), and could form a uniform thin layer on the ocular surface. Moreover, the drug release profile and gel dissolution rate revealed that the HA-MA network could retard the diffusion and dissolution of drug molecules and prolong the drug release time, which corresponded to an enhanced antibacterial ability of the HP-LFX gel. Furthermore, the HP gel exhibited low cytotoxicity to human corneal epithelial cells (HCECs). Finally, an in vivo pharmacodynamic study was conducted with rabbit keratitis models. An improved treatment efficacy was observed after application of the HP-LFX gels. This study highlights the potential of HP gels in ophthalmic drug delivery.
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Details
- Title
- A methacrylated hyaluronic acid network reinforced Pluronic F-127 gel for treatment of bacterial keratitis
- Creators
- Anyang Wang - Tsinghua UniversityLina Dong - Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, University of California Berkeley Tsinghua-Berkeley Shenzhen Institute, Energy & Enviromental Building, room 1112, Shenzhen, 518055, CHINA.Zhongwei Guo - Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China, Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute, Shenzhen 518055, P.R. China, Shenzhen, 518055, CHINA.Wei Sun - Tsinghua UniversityShengli Mi - University Town of Shenzhen
- Publication Details
- Biomedical materials (Bristol), v 17(4), p45017
- Publisher
- Institute of Physics (IOP)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000802739900001
- Scopus ID
- 2-s2.0-85131222220
- Other Identifier
- 991019167560204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials