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A method for spatio-temporal (4-D) data representation in confocal microscopy: application to neuroanatomical plasticity
Journal article   Peer reviewed

A method for spatio-temporal (4-D) data representation in confocal microscopy: application to neuroanatomical plasticity

A Kriete and H J Wagner
Journal of microscopy (Oxford), v 169(Pt 1), pp 27-31
Jan 1993
PMID: 8445630

Abstract

Culture Techniques Synapses - physiology Synapses - ultrastructure Retina - anatomy & histology Dark Adaptation Retina - physiology Algorithms Animals Image Processing, Computer-Assisted Microscopy, Fluorescence - methods Fishes Light Color Perception Photoreceptor Cells - physiology
This paper describes a new method for data representation and visualization in four dimensions (three dimensions plus time). Sequential volumes, exhibiting morphological activity, are acquired non-invasively with a confocal scanning laser microscope, where each data set corresponds to a time sample. A pipelined processing includes packing of volumes and specific volume rendering techniques. Subsequent processing in HIS (hue, intensity, saturation)l colour space combines functional, packed images with shaded three-dimensional views. As a result, even subtle changes in morphology become visible and computational time is saved. Experimental findings obtained from investigations of synaptic plasticity in cultured retinal tissue are reported.

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Collaboration types
Domestic collaboration
Web of Science research areas
Microscopy
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