Journal article
A modified histoimmunochemistry-assisted method for in situ RPE evaluation
Frontiers in bioscience (Elite edition), v 4(4), pp 1571-1581
01 Jan 2012
PMID: 22201976
Abstract
We have developed a method for morphological evaluation of retinal pigment epithelium (RPE) with minimal perturbation of RPE-Bruch's membrane-choriocapillaris complex (RBCC). To prepare RBCC, the anterior segments of the eye were removed. The remaining eye cup was radially cut into 4 to 6 pieces from periphery to the optic nerve head. Each piece was carefully dissected into 3 parts: sclera, RBCC and neurosensory retina. RBCC flatmount with RPE monolayer facing up was formed by several relaxing radial cuts. After immuno-staining with tissue specific markers, the RBCC could be distinguished as the superficial RPE monolayer and the underneath choriocapillaris layers by fluorescence microscopy. The density, distribution, and morphology of RPE cells varied among species. This method may have brought several advantages for RPE screening over other means, because of its straight forward approach, minimal manipulation of samples, plus there is no requirement for bleaching, which result in high efficiency for result readout. For a complete morphological study of RPE in situ, this method may be combined with other methods, such as cryosections, scanning electron microscopy, etc.
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Details
- Title
- A modified histoimmunochemistry-assisted method for in situ RPE evaluation
- Creators
- Jingfa Zhang - Department of Ophthalmology, Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, ChinaGuoxu XuLimei ZhangLimin GuHua XuLixia LuJuan WangFurong GaoJing-Ying XuFang WangWeiye LiGuo-Tong Xu
- Publication Details
- Frontiers in bioscience (Elite edition), v 4(4), pp 1571-1581
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Scopus ID
- 2-s2.0-84860849878
- Other Identifier
- 991019299008204721