Journal article
A multichamber fluidic device for 3D cultures under interstitial flow with live imaging: development, characterization, and applications
Biotechnology and bioengineering, v 105(5), pp 982-991
01 Apr 2010
PMID: 19953672
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Interstitial flow is an important biophysical cue that can affect capillary morphogenesis, tumor cell migration, and fibroblast remodeling of the extracellular matrix, among others. Current models that incorporate interstitial flow and that are suitable for live imaging lack the ability to perform multiple simultaneous experiments, for example, to compare effects of growth factors, extracellular matrix composition, etc. We present a nine-chamber radial flow device that allows simultaneous 3D fluidic experiments for relatively long-term culture with live imaging capabilities. Flow velocity profiles were characterized by fluorescence recovery after photobleaching (FRAP) for flow uniformity and estimating the hydraulic conductivity. We demonstrate lymphatic and blood capillary morphogenesis in fibrin gels over 10 days, comparing flow with static conditions as well as the effects of an engineered variant of VEGF that binds fibrin via Factor XIII. We also demonstrate the culture of contractile fibroblasts and co-cultures with tumor cells for modeling the tumor microenvironment. Therefore, this device is useful for studies of capillary morphogenesis, cell migration, contractile cells like fibroblasts, and multicellular cultures, all under interstitial flow.
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Details
- Title
- A multichamber fluidic device for 3D cultures under interstitial flow with live imaging: development, characterization, and applications
- Creators
- Carmen Bonvin - Institute of Bioengineering, School of Life Sciences/LMBM/Station 15, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne 1015, SwitzerlandJan OverneyAdrian C ShiehJ Brandon DixonMelody A Swartz
- Publication Details
- Biotechnology and bioengineering, v 105(5), pp 982-991
- Publisher
- Wiley; United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000275832200014
- Scopus ID
- 2-s2.0-77949408476
- Other Identifier
- 991014878049904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology