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Journal article
A naloxonazine sensitive (mu(1) receptor) mechanism in the parabrachial nucleus modulates eating
Brain research, v 1240, pp 111-118
13 Nov 2008
PMID: 18805404
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The parabrachial nucleus (PBN) is an area of the brain stem that controls eating and contains endogenous opioids and their receptors. Previously, we demonstrated that acute activation of mu opioid receptors (MOPR) in the lateral PBN increased food consumption. MOPRs have been divided operationally into mu(1) and mu(2) receptor subtypes on the basis of the ability of naloxonazine (Nlxz) to block the former but not the latter. We used autoradiography to measure whether Nlxz blocks stimulation by the mu(1)/mu(2) agonist DAMGO (D-Ala2, N-Me-Phe4, Gly5-ol-enkephalin) of the incorporation of [S-35]-guanosine 5'(gamma-thio)triphosphate([S-35]-GTP gamma S) into sections of the PBN. In vitro, Nlxz dose dependently inhibited receptor coupling in all areas of the PBN. The 1 mu M concentration of Nlxz reduced stimulation by 93.1 +/- 5% in the lateral inferior PBN (LPBNi) and by 90.5 +/- 4% in the medial parabrachial subregion (MPBN). Administration of Nlxz directly into the LPBNi decreased both food intake and agonist stimulated coupling, ex vivo, for the 24-h period after infusion. Infusion of Nlxz into the intended area reduced food intake by 42.3% below baseline values. Nlxz infusion prevented DAMGO stimulation of G-protein coupling in LPBNi and markedly reduced this stimulation in the MPBN. The incomplete inhibition of DAMGO-stimulated coupling in the MPBN is most likely due to the limited diffusion of Nlxz from the site of infusion (LPBNi) into this brain region. In conclusion, this study demonstrates that the mu(1) opioid receptor subtype is present in the parabrachial nucleus of the pons and that these receptors serve to modulate feeding in rats. (C) 2008 Elsevier B.V. All rights reserved.
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Details
- Title
- A naloxonazine sensitive (mu(1) receptor) mechanism in the parabrachial nucleus modulates eating
- Creators
- Nayla N. Chaijale - Drexel UniversityVincent J. Aloyo - Drexel UniversityKenny J. Simansky - Drexel University
- Publication Details
- Brain research, v 1240, pp 111-118
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- DK067648 / National Institute of Diabetes and Digestive and Kidney Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R01DK067648 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000261415600014
- Scopus ID
- 2-s2.0-54549120172
- Other Identifier
- 991019167450004721
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- Neurosciences