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A new procedure for experimental autoimmune uveitis with small uveitogenic peptides
Journal article   Peer reviewed

A new procedure for experimental autoimmune uveitis with small uveitogenic peptides

Theodore W Sery, Ying-Hsiu Su, Ralph Eagle, Jr, Masumi Ueda and Nobuto Yamamoto
Ocular immunology and inflammation, v 14(5)
Oct 2006
DOI: https://doi.org/10.1080/09273940600899684
PMID: 17056461
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Animals Autoimmune Diseases - chemically induced Autoimmune Diseases - immunology Autoimmune Diseases - pathology Eye Proteins - immunology Immunoconjugates - toxicity Macrophage Activation Models, Animal Peptide Fragments - immunology Pineal Gland - drug effects Pineal Gland - immunology Pineal Gland - pathology Rats Rats, Inbred Lew Retina - drug effects Retina - immunology Retina - pathology Retinol-Binding Proteins - immunology Streptococcus pyogenes - immunology Uvea - drug effects Uvea - immunology Uvea - pathology Uveitis - chemically induced Uveitis - immunology Uveitis - pathology
Demonstration of experimental autoimmune uveitis (EAU) with extremely small, fragmented peptides (12-30 amino acid residues) of interphotoreceptor retinoid-binding protein (IRPB). Very small fragmented peptides (no. 854, 888, 907, and 1057) were conjugated to heat-killed Group A Streptococcus cells and administered as a single intravenous injection to Lewis rats. A non-uveitogenic peptide 950 was also conjugated to heat-killed Streptococcus and administered. Administration of a mixture of small peptides and Streptococcus was a control for the peptides conjugated with Streptococcus. The uveitogenic peptide/Streptococcus conjugates produced uveitis inflammatory responses in the uvea, retina and pineal gland. Administration of mixtures of small peptides and Streptococcus cells, and a non-uveitogenic peptide 950 conjugated with Streptococcus did not produce autoimmune uveitis. Since mixtures of small uveitogenic peptides and Streptococcal cells did not develop autoimmune uveitis, conjugated Streptococcal cells provided a vehicle for macrophage phagocytosos of very small uveitogenic IRBP peptides. Subsequent antigen presentation from macrophages to lymphocytes developed autoimmune uveitis. Peptide 888, one of four IRBP peptides that encompass the major uveitogenic domain, proved to be the most effective in development of uveitis.

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Ophthalmology
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