Journal article
A new procedure for experimental autoimmune uveitis with small uveitogenic peptides
Ocular immunology and inflammation, Vol.14(5)
Oct 2006
PMID: 17056461
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Demonstration of experimental autoimmune uveitis (EAU) with extremely small, fragmented peptides (12-30 amino acid residues) of interphotoreceptor retinoid-binding protein (IRPB).
Very small fragmented peptides (no. 854, 888, 907, and 1057) were conjugated to heat-killed Group A Streptococcus cells and administered as a single intravenous injection to Lewis rats. A non-uveitogenic peptide 950 was also conjugated to heat-killed Streptococcus and administered. Administration of a mixture of small peptides and Streptococcus was a control for the peptides conjugated with Streptococcus.
The uveitogenic peptide/Streptococcus conjugates produced uveitis inflammatory responses in the uvea, retina and pineal gland. Administration of mixtures of small peptides and Streptococcus cells, and a non-uveitogenic peptide 950 conjugated with Streptococcus did not produce autoimmune uveitis.
Since mixtures of small uveitogenic peptides and Streptococcal cells did not develop autoimmune uveitis, conjugated Streptococcal cells provided a vehicle for macrophage phagocytosos of very small uveitogenic IRBP peptides. Subsequent antigen presentation from macrophages to lymphocytes developed autoimmune uveitis. Peptide 888, one of four IRBP peptides that encompass the major uveitogenic domain, proved to be the most effective in development of uveitis.
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Details
- Title
- A new procedure for experimental autoimmune uveitis with small uveitogenic peptides
- Creators
- Theodore W Sery - Wills Eye HospitalYing-Hsiu Su - Drexel UniversityRalph Eagle, JrMasumi Ueda - Tottori UniversityNobuto Yamamoto - Socrates Institute for Therapeutic Immunology, Philadelphia, PA, USA
- Publication Details
- Ocular immunology and inflammation, Vol.14(5)
- Publisher
- Taylor and Francis
- Grant note
- EY 2856 / NEI NIH HHS NS 35768-11 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019167455804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Ophthalmology