Journal article
A nickel-binding serpin, pNiXa, induces maturation of Xenopus oocytes and shows synergism with oncogenic ras-p21 protein
Research communications in chemical pathology and pharmacology, v 79(2), pp 131-140
Feb 1993
PMID: 8451539
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A nickel-binding serine proteinase inhibitor, pNiXa (43 kDa), was isolated from Xenopus ovary and assayed for effects on oocyte maturation. Microinjection of pNiXa (0.12 pmol/50 nl) induced maturation in 60% of Xenopus oocytes, beginning at 4 hours and reaching completion by 9 hours. Microinjection of oncogenic ras-p21 protein (0.12 pmol/50 nl) induced maturation in 79% of oocytes, beginning at 6 hours and reaching completion by 12 hours. Microinjection of pNiXa in combination with ras-p21 protein had a synergistic effect on maturation, which occurred in 92% of oocytes, beginning at 4 hours and reaching completion by 9 hours. Oocyte maturation did not occur in control oocytes, which received a microinjection of bovine serum albumin. In oocytes exposed to a combination of pNiXa (0.12 pmol/50 nl, by microinjection) and progesterone (10 micrograms/ml, in the medium), maturation was intermediate (68% at 9 hours) between that induced by pNiXa (60%) or progesterone (85%) alone. This study shows (a) that pNiXa is a potent inducer of oocyte maturation, (b) that pNiXa's effect is synergistic with that of oncogenic ras-p21 protein, and (c) that pNiXa partially antagonizes progesterone induction of oocyte maturation.
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Details
- Title
- A nickel-binding serpin, pNiXa, induces maturation of Xenopus oocytes and shows synergism with oncogenic ras-p21 protein
- Creators
- J Haspel - Department of Pathology, SUNY Health Science Center, Syracuse 13210F W Sunderman, JrS M HofperD C HenjumP W Brandt-RaufI B WeinsteinS NishimuraZ YamaizumiM R Pincus
- Publication Details
- Research communications in chemical pathology and pharmacology, v 79(2), pp 131-140
- Grant note
- ES-05331 / NIEHS NIH HHS CA-02111 / NCI NIH HHS CA-42500 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:A1993KP20500001
- Scopus ID
- 2-s2.0-0027456445
- Other Identifier
- 991019342443204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Pathology
- Pharmacology & Pharmacy