Journal article
A novel fragment of antigen binding (Fab) surface display platform using glycoengineered Pichia pastoris
Journal of immunological methods, v 375(1-2), 159
31 Jan 2012
PMID: 22019510
Abstract
Afragment of antigen binding (Fab) surface display system was developed using a glycoengineered Pichia pastoris host strain genetically modified to secrete glycoproteins with mammalian mannose-type Man(5)GlcNAc(2) N-linked glycans. The surface display method described here takes advantage of a pair of coiled-coil peptides as the linker while using the Saccharomyces cerevisiae Sed1p GPI-anchored cell surface protein as an anchoring domain. Several Fabs were successfully displayed on the cell surface using this system and the expression level of the displayed Fabs was correlated to that of secreted Fabs from the same glycoengineered host in the absence of the cell wall anchor. Strains displaying different model Fabs were mixed and, through cell sorting, the strain displaying more expressed Fab molecule or the strain displaying the Fab with higher affinity for an antigen was effectively enriched by FACS. This novel yeast surface display system provides a general platform for the display of Fab libraries for affinity and/or expression maturation using glycoengineered Pichia. (C) 2011 Elsevier B.V. All rights reserved.
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Details
- Title
- A novel fragment of antigen binding (Fab) surface display platform using glycoengineered Pichia pastoris
- Creators
- Song Lin - GlycoFi Inc, Lebanon, NH 03766 USANga Rewa Houston-Cummings - Merck & Co., Inc., Rahway, NJ, USA (United States)Bianka Prinz - Merck & Co., Inc., Rahway, NJ, USA (United States)Renee Moore - Merck & Co., Inc., Rahway, NJ, USA (United States)Beata Bobrowicz - Merck & Co., Inc., Rahway, NJ, USA (United States)Robert C. Davidson - Merck & Co., Inc., Rahway, NJ, USA (United States)Stefan Wildt - Merck & Co., Inc., Rahway, NJ, USA (United States)Terrance A. Stadheim - Merck & Co., Inc., Rahway, NJ, USA (United States)Dongxing Zha - Merck & Co., Inc., Rahway, NJ, USA (United States)
- Publication Details
- Journal of immunological methods, v 375(1-2), 159
- Publisher
- Elsevier
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Epidemiology and Biostatistics
- Web of Science ID
- WOS:000300913300019
- Scopus ID
- 2-s2.0-84855346757
- Other Identifier
- 991021448180904721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Immunology