Journal article
A novel method for the direct fabrication of growth factor-loaded microspheres within porous nondegradable hydrogels: Controlled release for cartilage tissue engineering
Journal of controlled release, v 157(1), pp 39-45
10 Jan 2012
PMID: 21930167
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Because of similar mechanical properties to native cartilage, synthetic hydrogels based on poly(vinyl alcohol) (PVA) have been proposed for replacement of damaged articular cartilage, but they suffer from a complete lack of integration with surrounding tissue. In this study, insulin-like growth factor-1 (IGF-1), an important growth factor in cartilage regeneration, was encapsulated in degradable poly(lactic-co-glycolic acid) (PLGA) microparticles embedded in the PVA hydrogels in a single step based on a double emulsion. The release of IGF-1 from these hydrogels was sustained over 6 weeks in vitro. Poly(glycolic acid) (PGA) fiber scaffolds were wrapped around the hydrogels, seeded with chondrocytes, and implanted subcutaneously in athymic mice. The release of IGF-1 enhanced cartilage formation in the layers surrounding the hydrogels, in terms of the content of extracellular matrix components and mechanical properties, and increased integration between the cartilage layers and the hydrogels, according to gross observation of the cross-sections and histology. The compressive modulus of the cartilage-hydrogel constructs without IGF-1 was 0.07 +/- 0.02 MPa, compared to 0.17-0.2 MPa for hydrogels that contained IGF-1. The biochemical and mechanical markers of cartilage formation were not different between the low and high concentrations of IGF-1, despite an order of magnitude difference in concentration. This study shows that the sustained release of IGF-1 can enhance tissue formation and points to a possible strategy for effecting integration with surrounding tissue. (C) 2011 Elsevier B. V. All rights reserved.
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Details
- Title
- A novel method for the direct fabrication of growth factor-loaded microspheres within porous nondegradable hydrogels: Controlled release for cartilage tissue engineering
- Creators
- Kara L. Spiller - Drexel UniversityYu Liu - Shanghai Jiao Tong UniversityJulianne L. Holloway - Drexel UniversitySuzanne A. Maher - Hospital for Special SurgeryYilin Cao - Shanghai Jiao Tong UniversityWei Liu - Shanghai Jiao Tong UniversityGuangdong Zhou - Shanghai Jiao Tong UniversityAnthony M. Lowman - Drexel University
- Publication Details
- Journal of controlled release, v 157(1), pp 39-45
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- 50830105; 30973131; 30973130; 81000677 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 08SG19 / Shanghai Aurora Program 09QH1401600 / Shanghai Rising-star Program U.S. National Science Foundation; National Science Foundation (NSF) 0935871 / Doctoral Dissertation Enhancement Program 2006AA02A126 / Hi-Tech Research and Development Program of China; National High Technology Research and Development Program of China 2005CB522702; 2010CB944804 / National Basic Research Program of China
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000300775700005
- Scopus ID
- 2-s2.0-84855842640
- Other Identifier
- 991019168518104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Pharmacology & Pharmacy