Journal article
A novel partnership between lncTCF7 and SND1 regulates the expression of the TCF7 gene via recruitment of the SWI/SNF complex
Scientific reports, v 14(1), p19384
21 Aug 2024
PMID: 39169000
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Long non-coding RNAs (lncRNAs) play key roles in cellular pathways and disease progression, yet their molecular mechanisms remain largely understudied. The lncRNA lncTCF7 has been shown to promote tumor progression by recruiting the SWI/SNF complex to the TCF7 promoter, activating its expression and the WNT signaling pathway. However, how lncTCF7 recruits SWI/SNF remains to be determined, and lncTCF7-specific binding partners are unknown. Using RNA-pulldown and quantitative mass spectrometry, we identified a novel interacting protein partner for lncTCF7, SND1, a multifunctional RNA binding protein that can also function as a transcription co-activator. Knockdown analysis of lncTCF7 and SND1 reveals that they are both required for the expression of TCF7. Chromatin immunoprecipitation assays suggest that both SND1 and lncTCF7 are required for recruiting the SWI/SNF chromatin remodeling complex, and these functions, in tandem, activate the expression of TCF7. Finally, using structural probing and RNA-pulldown of lncTCF7 and its subdomains, we highlight the potential binding region for SND1 in the 3’-end of lncTCF7. Overall, this study highlights the critical roles lncRNAs play in regulating gene expression and provides new insights into the complex network of interactions that underlie this process.
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Details
- Title
- A novel partnership between lncTCF7 and SND1 regulates the expression of the TCF7 gene via recruitment of the SWI/SNF complex
- Creators
- Allison Yankey - Drexel UniversityMihyun Oh - Drexel UniversityBo Lim Lee - Drexel UniversityTisha Kalpesh Desai - Drexel UniversitySrinivas Somarowthu - Drexel University
- Publication Details
- Scientific reports, v 14(1), p19384
- Publisher
- Nature Publishing Group UK
- Number of pages
- 13
- Grant note
We thank Dr. Mauricio Reginato and Dr. Jane Clifford at Drexel University College of Medicine for reagents and Samuel Evans for technical assistance.DAS:The data underlying this article are available in the article and in its online supplementary material.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001296167300074
- Scopus ID
- 2-s2.0-85201660347
- Other Identifier
- 991021899175704721
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- Web of Science research areas
- Biochemistry & Molecular Biology