Journal article
A novel pulsed-plasma approach for protein immobilization by grafting reactive amine groups on polyurethane-coated biosensors
Sensors and actuators. B, Chemical, v 173, pp 569-574
Oct 2012
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Reliable surface chemistry is necessary for immobilization of biological recognition molecules on sensor surfaces. A dry and rapid method of pulsed-plasma assisted grafting of amine surface functional groups, using ammonia gas on polyurethane surfaces is successfully demonstrated by using pulse cycle times of 1–180s (on–off). One cycle of plasma treatment was found to generate higher amine groups as they tend to degrade upon longer exposure to the high-energy radicals. Successful amination of the polyurethane surfaces was demonstrated by Fourier transform infrared spectroscopy (FTIR). To establish the availability of the surface amine groups for reaction, 1μm carboxylated polybeads were reacted to polyurethane-coated piezoelectric excited millimeter-sized cantilever (PEMC) sensors before and after plasma treatment. Scanning electron microscope micrographs of PEMC sensors exposed to the same concentration of beads show more than four-fold increase in the presence of beads post pulsed-plasma treatment. Application of this method for protein immobilization was established by BSA chemisorption on plasma treated PEMC sensors.
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Details
- Title
- A novel pulsed-plasma approach for protein immobilization by grafting reactive amine groups on polyurethane-coated biosensors
- Creators
- Harsh SharmaRaj Mutharasan
- Publication Details
- Sensors and actuators. B, Chemical, v 173, pp 569-574
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000311248100080
- Scopus ID
- 2-s2.0-84867045873
- Other Identifier
- 991014877999504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation