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Journal article
A novel role for WAVE1 in controlling actin network growth rate and architecture
Molecular biology of the cell, v 26(3), pp 495-505
01 Feb 2015
PMID: 25473116
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Branched actin filament networks in cells are assembled through the combined activities of Arp2/3 complex and different WASP/WAVE proteins. Here we used TIRF and electron microscopy to directly compare for the first time the assembly kinetics and architectures of actin filament networks produced by Arp2/3 complex and dimerized VCA regions of WAVE1, WAVE2, or N-WASP. WAVE1 produced strikingly different networks from WAVE2 or N-WASP, which comprised unexpectedly short filaments. Further analysis showed that the WAVE1-specific activity stemmed from an inhibitory effect on filament elongation both in the presence and absence of Arp2/3 complex, which was observed even at low stoichiometries of WAVE1 to actin monomers, precluding an effect from monomer sequestration. Using a series of VCA chimeras, we mapped the elongation inhibitory effects of WAVE1 to its WH2 ("V") domain. Further, mutating a single conserved lysine residue potently disrupted WAVE1's inhibitory effects. Taken together, our results show that WAVE1 has unique activities independent of Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks. Such activities may underlie previously observed differences between the cellular functions of WAVE1 and WAVE2.
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Details
- Title
- A novel role for WAVE1 in controlling actin network growth rate and architecture
- Creators
- Meredith O Sweeney - Brandeis UniversityAgnieszka Collins - Brandeis UniversityShae B Padrick - The University of Texas Southwestern Medical CenterBruce L Goode
- Publication Details
- Molecular biology of the cell, v 26(3), pp 495-505
- Grant note
- GM063691 / NIGMS NIH HHS R01 GM063691 / NIGMS NIH HHS R01-GM56322 / NIGMS NIH HHS Howard Hughes Medical Institute R01 GM056322 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000348857300010
- Scopus ID
- 2-s2.0-84921847750
- Other Identifier
- 991020836339304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology