Journal article
A novel sucrose porogen-based solid freeform fabrication system for bone scaffold manufacturing
Rapid prototyping journal, v 16(5), pp 365-376
03 Aug 2010
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Purpose - Musculoskeletal conditions are a major health concern in the USA because of a large aging population and increased occurrence of sport-related injuries. Bone tissue engineering may offer a less painful alternative to traditional bone grafts with lower risk of infection. The purpose of this paper is to present a novel porogen-based fabrication system for tissue engineering scaffolds using sucrose (C12H22O11) as the porogen building material.Design methodology approach - A new solid freeform fabrication system has been developed and tested, which uses pressurized extrusion to print highly biocompatible and water soluble sucrose bone scaffold porogens (or negtives). Polycaprolactone (PCL) scaffolds are manufactured by injecting molten polymer into the porogens, and the porogens are subsequently dissolved with water. The resultant scaffolds demonstrate the defined porous structure designed into the sucrose porogen manufacturing computer-aided design model.Findings - To optimize the porogen manufacturing process, the viscosity of sucrose mixtures is measured. Design of experiments is used to plan and analyze the relationships between the porogen characteristics and the process parameters. Reservoir pressure and print head speed are identified as the dominant factors affecting sucrose flow rate and porogen strut diameter, respectively. The biocompatibility of the new system is assessed by in vitro cell culture testing. Endothelial hybridoma cells (EAhy 926) and osteoblasts (7F2) seeded on the fabricated PCL scaffolds adhered to the scaffold and proliferated over four to six days. Epifluorescence and scanning electron microscopy images reveal cell spreading and multiple layers of cells on the scaffold surface. The results demonstrated the potential of the structured sucrose porogen-based fabrication method in manufacturing bone tissue scaffolds.Originality value - This paper describes the first time use of biomaterials-sucrose to make scaffold porogens and how an injection molded biopolymer scaffold can then be received.
Metrics
Details
- Title
- A novel sucrose porogen-based solid freeform fabrication system for bone scaffold manufacturing
- Creators
- Lin Lu - Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USAQingwei Zhang - Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USADavid Wootton - Department of Mechanical Engineering, Cooper Union, New York, USAPeter I Lelkes - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania, USAJack Zhou - Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USA
- Publication Details
- Rapid prototyping journal, v 16(5), pp 365-376
- Publisher
- Emerald Group Publishing Limited
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000281178800008
- Scopus ID
- 2-s2.0-77954978492
- Other Identifier
- 991014878222604721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Mechanical
- Materials Science, Multidisciplinary