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A novel total knee arthroplasty infection model in rabbits
Journal article   Open access   Peer reviewed

A novel total knee arthroplasty infection model in rabbits

Matthew Craig, Kornelis Poelstra, JChristopher Sherrell, Michael Kwon, Etienne Belzile and Thomas Brown
Journal of orthopaedic research, v 23(5), pp 1100-1104
Sep 2005
DOI: https://doi.org/10.1016/j.orthres.2005.03.007
PMID: 15927441
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1016/j.orthres.2005.03.007View
Published, Version of Record (VoR) Open

Abstract

Staphylococcus aureus
Infection of biomaterial implants is an expensive and devastating complication of orthopaedic surgery historically ranging from less than 1% in primary total knee arthroplasty (TKA) to 10% in revision TKA. An in vivo animal model was developed to test the efficacy of innovative therapies for the prevention of biomaterial centered infections caused by methicillin-resistant Staphylococcus aureus bacteria (MRSA). Twenty-two New Zealand White rabbits were used in this study. After proper anesthesia, a stainless-steel screw with a high molecular weight polyethylene (UHMWPE) washer was cemented in a defect created in the intra-articular, non-articulating portion of the lateral femoral condyle of each knee. After closure of the joint capsule, each knee was inoculated with 0, 10 super(2), 10 super(3), or 10 super(4) colony forming units (CFU) of MRSA. Animals were sacrificed after 7 days at which time joint aspirate, tissues and biomaterial samples were examined for evidence of infection. A total of 42 knees were used for analysis. When saline was injected into the knee, 0/10 of the knees demonstrated evidence of biomaterial centered infection (with the contralateral knee receiving 10 super(4) CFU MRSA). Four of 10 knees developed a biomaterial centered infection when 10 super(2) CFU MRSA was introduced. Seven out of 10 knees developed a biomaterial centered infection when either 10 super(3) or 10 super(4) CFU MRSA was injected. No evidence of septicemia (positive blood cultures) was found in any animal. This rabbit knee model utilizes commonly employed inexpensive orthopaedic implant materials in an in vivo milieu and provides an effective method for the evaluation of treatments for biomaterial centered infections.

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Domestic collaboration
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Web of Science research areas
Orthopedics
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