Journal article
A plasma separator with a multifunctional deformable chamber equipped with a porous membrane for point-of-care diagnostics
Analyst (London), v 145(18), pp 6138-6147
14 Sep 2020
PMID: 32869771
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Traditionally, plasma is extracted from whole blood using centrifuges in clinical laboratories, which is unsuitable for on-site testing. For point-of-care diagnostics, for example in HIV tests, to ensure the detection sensitivity for low-abundance analytical targets, a relatively large volume of plasma needs to be extracted from milliliters of blood with a simpler and easier-to-operate method than centrifugation. We report the development of a membrane-assisted, sedimentation-facilitated plasma separator with a multifunctional deformable chamber, which is able to perform plasma separation from undiluted whole blood in a short time. Multiple steps related to plasma separation, including cell sedimentation, cell filtration, and plasma driving and discharging, are all performed in or through the multifunctional deformable chamber equipped with a top-layer porous membrane, which significantly reduces the device complexity. Assisted by a simple jig or even hands, plasma separation can be conveniently performed upon mechanical actuation of the deformable chamber. Within 8 min, similar to 130 mu L of plasma can be conveniently extracted with the described device from 2.3 mL of whole blood. It has been demonstrated that HIV antibodies or virus spiked in whole blood can be successfully detected with reasonable sensitivity from the extracted plasma with the described pump-free device.
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Details
- Title
- A plasma separator with a multifunctional deformable chamber equipped with a porous membrane for point-of-care diagnostics
- Creators
- Xianbo Qiu - Beijing University of Chemical TechnologyHuiqin Jiang - Beijing University of Chemical TechnologyXiaolei Zhang - Beijing Wantai Biological Pharmacy (China)Ke Li - Beijing Wantai Biological Pharmacy (China)Shengxiang Ge - Xiamen UniversityNingshao Xia - Xiamen UniversityMichael G. Mauk - Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USA
- Publication Details
- Analyst (London), v 145(18), pp 6138-6147
- Publisher
- Royal Soc Chemistry
- Number of pages
- 10
- Grant note
- 81371711; 81871505 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) XK1802-4; PYBZ1830; PT1908 / Fundamental Research Funds for the Central Universities 2018ZX10732101-001-009 / National Science and Technology Major Project buctylkjcx06 / Research Fund to the Top Scientific and Technological Innovation Team from Beijing University of Chemical Technology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000568961600016
- Scopus ID
- 2-s2.0-85090883404
- Other Identifier
- 991020623758704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical