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Journal article
A portable, 3D printed, microfluidic device for multiplexed, real time, molecular detection of the porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacoronavirus at the point of need
Lab on a chip, v 21(6), pp 1118-1130
21 Mar 2021
PMID: 33527920
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV) are emerging/reemerging coronaviruses (CoVs) of neonatal pigs that cause great economic losses to pig farms and pork processors. Specific, rapid, and simple multiplex detection of these viruses is critical to enable prompt implementation of appropriate control measures. Conventional methods for molecular diagnosis require skilled personnel and relatively sophisticated equipment, restricting their use in centralized laboratories. We developed a low-cost, rapid, semi-quantitative, field deployable, 3D-printed microfluidic device for auto-distribution of samples and self-sealing and real-time and reverse transcription-loop-mediated isothermal amplification (RT-LAMP), enabling the co-detection of PEDV, TGEV and PDCoV within 30 minutes. Our assay's analytical performance is comparable with a benchtop, real-time RT-LAMP assay and the gold standard quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay with limits of detection of 10 genomic copies per reaction for PEDV and PDCoV, and 100 genomic copies per reaction for TGEV. Evaluation of clinical specimens from diseased pigs with our microfluidic device revealed excellent concordance with both benchtop RT-LAMP and qRT-PCR. Our portable RT-LAMP microfluidic chip will potentially facilitate simple, specific, rapid multiplexed detection of harmful infections in minimally equipped veterinary diagnostic laboratories and on-site in pigs' farms.
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Details
- Title
- A portable, 3D printed, microfluidic device for multiplexed, real time, molecular detection of the porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacoronavirus at the point of need
- Creators
- Mohamed El-Tholoth - University of Veterinary MedicineHuiwen Bai - California University of PennsylvaniaMichael G. Mauk - Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USALinda Saif - Département Santé AnimaleHaim H. Bau - California University of Pennsylvania
- Publication Details
- Lab on a chip, v 21(6), pp 1118-1130
- Publisher
- Royal Soc Chemistry
- Number of pages
- 13
- Grant note
- 1R21AI128059 / NIH of the University of Pennsylvania; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Fulbright Visiting Scholar Program
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000632163600007
- Scopus ID
- 2-s2.0-85103254801
- Other Identifier
- 991020623759804721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Multidisciplinary
- Instruments & Instrumentation
- Nanoscience & Nanotechnology