Journal article
A possible dual physiological role of extracellular ATP in the modulation of platelet aggregation
Biochimica et biophysica acta. Molecular cell research, v 1355(2), pp 131-140
1997
PMID: 9042333
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ATP and ADP are simultaneously released from activated platelets in equimolar concentrations. Micromolar concentrations of ATP inhibit platelet aggregation by both competitive and non-competitive mechanisms. The current studies addressed the question of how platelets respond to agonists in the presence of nanomolar and micromolar concentrations of ATP and ADP alone or in combination. This is a significant issue since the concentration of ATP±ADP may vary widely within a microenvironment depending upon the source and cause for the release of the nucleotides. ATP (1–10 nM) was found to significantly enhance the thromboxane A
2 analog, U44619-, collagen- and thrombin-induced platelet aggregations. Conversely, ATP at 1–100
μM inhibited these same reactions. ADP, in general, behaved exactly opposite to ATP. When equal amounts of ATP and ADP were added together the ADP response appeared to predominate. The observed ATP-induced response was not due to a hydrolytic product as evidenced by an unaltered response to ATP in the presence of adenosine deaminase or the ATP generating system, creatine phosphate plus creatine phosphokinase. Adenosine (1–10 nM), like ADP, inhibited agonist-induced platelet aggregation. The stimulation of agonist-induced platelet aggregation by 1–10 nM extracellular ATP appears to depend upon the phosphorylation of platelet membrane ecto proteins. The ATP analog,
βγ-methylene ATP, that is incapable of serving as a phosphate donor for protein kinases, inhibited rather than stimulated agonist-induced platelet aggregation. The dual response of platelets to low and high concentrations of extracellular ATP±ADP may play a physiological role in hemostasis and thrombosis under normal and pathological conditions.
Metrics
Details
- Title
- A possible dual physiological role of extracellular ATP in the modulation of platelet aggregation
- Creators
- Gerald Soslau - Department of Biochemistry, Medical College of Pennsylvania and Hahnemann University, Philadelphia, PA 19102-1192, USADonya Youngprapakorn - Division of Neoplastic Diseases, Medical College of Pennsylvania and Hahnemann University, Philadelphia, PA 19102-1192, USA
- Publication Details
- Biochimica et biophysica acta. Molecular cell research, v 1355(2), pp 131-140
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1997WG59100006
- Scopus ID
- 2-s2.0-0031058520
- Other Identifier
- 991014877662604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology