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A reagentless electrochemical biosensor based on a protein scaffold
Journal article

A reagentless electrochemical biosensor based on a protein scaffold

Sulay D Jhaveri, J Matthew Mauro, Harold M Goldston, Caroline L Schauer, Leonard M Tender and Scott A Trammell
Chemical communications (Cambridge, England), v 9(3), pp 338-339
07 Feb 2003
PMID: 12613601

Abstract

Apoproteins - chemistry Molecular Probe Techniques Oxidation-Reduction Myoglobin - chemistry Ruthenium - chemistry Biotin - chemistry Electrodes Organometallic Compounds Animals Whales Protein Engineering Electrochemistry Avidin - chemistry Biosensing Techniques - methods
Apo-myoglobin, labeled with the environmentally sensitive redox probe RuII(NH3)4(1,10-phenanthroline-5-maleimide)2+, was immobilized onto gold electrodes modified with 11-mercaptoundecanoic acid and subsequently labeled with biotin; avidin binding to the immobilized biotin was specifically and quantitatively detected by a change in cyclic voltammetry of the co-immobilized probe.

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Web of Science research areas
Chemistry, Multidisciplinary
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