Journal article
A role for tissue transglutaminase in hepatic injury and fibrogenesis, and its regulation by NF-kappaB
The American journal of physiology, v 272(2 Pt 1), pp G281-G288
Feb 1997
PMID: 9124352
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study was undertaken to delineate a possible role for tissue transglutaminase (tTG), an enzyme that catalyzes protein cross-linking, in hepatic fibrogenesis. Rats were treated with CCl4 solution and then killed at different stages of liver injury and fibrogenesis. Liver tTG mRNA levels were markedly increased as early as 6 h after the first injection, peaked at 4 days and 1 wk, and remained increased for 8 wk. The enzymatic activity of tTG was increased in livers of rats treated with CCl4, in a fashion that paralleled the Northern blot results. Cell isolation experiments indicated that all hepatic cell types synthesize tTG mRNA. Increased binding to the nuclear factor-kappaB (NF-kappaB) motif of the tTG promoter was found in the nuclear extracts prepared from CCl4-treated samples. These data demonstrate an increase in tTG gene expression during hepatic injury and fibrosis, suggesting a possible role for this enzyme in stabilizing the fibrotic bands during hepatic fibrogenesis. Moreover, increased NF-kappaB binding to the tTG promoter may represent one of the mechanisms by which cell injury induces tTG transcription and thus potentiates the process of fibrogenesis.
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Details
- Title
- A role for tissue transglutaminase in hepatic injury and fibrogenesis, and its regulation by NF-kappaB
- Creators
- A Mirza - Department of Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USAS L LiuE FrizellJ ZhuS MaddukuriJ MartinezP DaviesR SchwartingP NortonM A Zern
- Publication Details
- The American journal of physiology, v 272(2 Pt 1), pp G281-G288
- Publisher
- American Physiological Society (APS); United States
- Grant note
- GM-46402 / NIGMS NIH HHS AA-06386 / NIAAA NIH HHS DK-41875 / NIDDK NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1997WJ80100010
- Scopus ID
- 2-s2.0-0030900790
- Other Identifier
- 991014878512704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Gastroenterology & Hepatology
- Physiology