Journal article
A sensitive CRISPR/Cas12a-assisted fluorescent aptasensor for rapid detection of food allergens
International journal of biological macromolecules, v 274(Pt 2), 133444
Aug 2024
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Food allergens elicit abnormal immune system responses among allergic individuals and sensitive detection for allergenic ingredient is greatly significant. To address this need, a novel fluorescent aptasensor, assisted by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), have been developed for food allergens. In this study, aptamer offers distinctive recognition capabilities in binding specific targets, while CRISPR-associated-12a protein (Cas12a) holds precise cis-cleavage for cutting fluorescent signal probes. Notably, the utilization of Cas12a cis-cleavage activity, rather than trans-cleavage, eliminates the necessity for additional fluorescent probes, thus reducing interference between substances and enhancing sensitivity. Throughout the process, complementary DNA (cDNA) plays a crucial dual role in target recognition conversion and signal presentation, representing a key challenge and innovative aspect of this study. To evaluate the performance of the aptasensor, lysozyme (LYS) is employed as a representative model target of food allergens. Under optimal conditions, the developed aptasensor could achieve an exceptional low limit of detection (LOD) of 6.10 pM with a dynamic detection range of 10 pM-320 pM. The aptasensor demonstrates high selectivity and great recovery rates. This strategy yields promising outcomes, holding the potential to serve as a valuable reference for various food allergens detection.
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Details
- Title
- A sensitive CRISPR/Cas12a-assisted fluorescent aptasensor for rapid detection of food allergens
- Creators
- Ling Li - Beijing University of Chemical TechnologyMenglei Li - Beijing University of Chemical TechnologyYinghua Qiu - Drexel UniversityYiyang Dong - Beijing University of Chemical Technology
- Publication Details
- International journal of biological macromolecules, v 274(Pt 2), 133444
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001262775400001
- Scopus ID
- 2-s2.0-85197060607
- Other Identifier
- 991021889866004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Applied
- Polymer Science