Journal article
A study of gas‐phase cationization in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Rapid communications in mass spectrometry, v 20(14), pp 2165-2169
30 Jul 2006
PMID: 16779867
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A specially constructed split sample probe was used to unequivocally demonstrate that gas‐phase cationization occurs within the desorption plume during a matrix‐assisted laser desorption/ionization experiment. Two separate samples were prepared for analysis: on side A, a mixture of poly(ethylene glycol) (PEG) 1500 analyte and 2,5‐dihydroxybenzoic acid (DHB) matrix, and on side B a mixture of DHB matrix and lithium hydroxide (LiOH), the cationization reagent. Analysis of the data showed that when the ionization laser was focused on the split (so that both sides were illuminated), Li+‐cationized PEG peaks were observed. Since the PEG analyte did not come into contact with Li+ in either the solution or solid phase, the only possibility for the observed cationization was a reaction in the gas phase. Due to the difficulty in completely removing the adventitious cations (Na+ and K+) present in DHB and on sample surfaces, gas‐phase cationization could not be demonstrated to be either the only or most important mechanism operating in the MALDI experiment. Copyright © 2006 John Wiley & Sons, Ltd.
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Details
- Title
- A study of gas‐phase cationization in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
- Creators
- William J ErbScott D HantonKevin G Owens
- Publication Details
- Rapid communications in mass spectrometry, v 20(14), pp 2165-2169
- Publisher
- John Wiley & Sons, Ltd; Chichester, UK
- Number of pages
- 5
- Grant note
- Air Products and Chemicals, Inc.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000239054700014
- Scopus ID
- 2-s2.0-33745885675
- Other Identifier
- 991014877816604721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Spectroscopy