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A styrene-alt-maleic acid copolymer is an effective inhibitor of R5 and X4 human immunodeficiency virus type 1 infection
Journal article   Open access

A styrene-alt-maleic acid copolymer is an effective inhibitor of R5 and X4 human immunodeficiency virus type 1 infection

Vanessa Pirrone, Shendra Passic, Brian Wigdahl, Robert F Rando, Mohamed Labib and Fred C Krebs
Journal of biomedicine & biotechnology, v 2010, 548749
2010
PMID: 20589074
url
https://doi.org/10.1155/2010/548749View
Published, Version of Record (VoR) Open

Abstract

Cell Survival - drug effects HIV Infections - prevention & control Antiviral Agents - pharmacology HIV-1 - pathogenicity Maleates - pharmacology HIV-1 - drug effects Humans Polystyrenes - pharmacology Cells, Cultured Virus Internalization - drug effects Leukocytes, Mononuclear - virology T-Lymphocytes - virology HIV-1 - classification Receptors, CCR5
An alternating copolymer of styrene and maleic acid (alt-PSMA) differs from other polyanionic antiviral agents in that the negative charges of alt-PSMA are provided by carboxylic acid groups instead of sulfate or sulfonate moieties. We hypothesized that alt-PSMA would have activity against human immunodeficiency virus type 1 (HIV-1) comparable to other polyanions, such as the related compound, poly(sodium 4-styrene sulfonate) (PSS). In assays using cell lines and primary immune cells, alt-PSMA was characterized by low cytotoxicity and effective inhibition of infection by HIV-1 BaL and IIIB as well as clinical isolates of subtypes A, B, and C. In mechanism of action assays, in which each compound was added to cells and subsequently removed prior to HIV-1 infection ("washout" assay), alt-PSMA caused no enhancement of infection, while PSS washout increased infection 70% above control levels. These studies demonstrate that alt-PSMA is an effective HIV-1 inhibitor with properties that warrant further investigation.

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Web of Science research areas
Biotechnology & Applied Microbiology
Medicine, Research & Experimental
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