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A temperature-sensitive mutant of the mammalian RNA helicase, DEAD-BOX X isoform, DBX, defective in the transition from G1 to S phase
Journal article   Peer reviewed

A temperature-sensitive mutant of the mammalian RNA helicase, DEAD-BOX X isoform, DBX, defective in the transition from G1 to S phase

Junko Fukumura, Eishi Noguchi, Takeshi Sekiguchi and Takeharu Nishimoto
Journal of biochemistry (Tokyo), v 134(1), pp 71-82
Jul 2003
DOI: https://doi.org/10.1093/jb/mvg126
PMID: 12944373
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Cyclin A - biosynthesis Temperature Humans DNA, Complementary - genetics Molecular Sequence Data Eukaryotic Initiation Factor-4E - biosynthesis Genetic Complementation Test RNA, Messenger - biosynthesis Sequence Analysis, Protein Cyclin B - biosynthesis Cell Nucleus - metabolism RNA Helicases - physiology Transfection RNA Helicases - genetics G1 Phase - genetics Amino Acid Sequence Cell Line Cricetinae Isoenzymes G1 Phase - physiology S Phase - genetics DNA - genetics Point Mutation DNA - chemistry Animals S Phase - physiology HeLa Cells Genome, Human
ts ET24 cells are a novel temperature-sensitive (ts) mutant for cell proliferation of hamster BHK21 cells. The human genomic DNA which rescued the temperature-sensitive lethality of ts ET24 cells was isolated and screened for an open reading frame in the deposited human genomic library. X chromosomal DBX gene encoding the RNA helicase, DEAD-BOX X isoform, which is homologous to yeast Ded1p, was found to be defective in this mutant. The single point mutation (P267S) was localized between the Motifs I and Ia of the hamster DBX of ts ET24 cells. At the nonpermissive temperature of 39.5 degrees C, ts ET24 cells were arrested in the G1-phase and survived for more than 3 days. In ts ET24 cells, total protein synthesis was not reduced at 39.5 degrees C for 24 h, while mRNA accumulated in the nucleus after incubation at 39.5 degrees C for 17 h. The amount of cyclin A mRNA decreased in ts ET24 cells within 4 h after the temperature shift to 39.5 degrees C, consistent with the fact that the entry into the S-phase was delayed by the temperature shift.

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Web of Science research areas
Biochemistry & Molecular Biology
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