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A translational cellular model to study the impact of high-frequency oscillatory ventilation on human epithelial cell function
Journal article   Open access   Peer reviewed

A translational cellular model to study the impact of high-frequency oscillatory ventilation on human epithelial cell function

Anja Mowes, Beatriz E de Jongh, Timothy Cox, Yan Zhu and Thomas H Shaffer
Journal of applied physiology (1985), v 122(1), pp 198-205
01 Jan 2017
DOI: https://doi.org/10.1152/japplphysiol.00400.2016
PMID: 27834669
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1152/japplphysiol.00400.2016View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open

Abstract

Cells, Cultured Epithelial Cells - metabolism Epithelial Cells - physiology High-Frequency Ventilation - methods Humans Hyperoxia - metabolism Hyperoxia - physiopathology Lung Injury - metabolism Lung Injury - physiopathology Oxygen - metabolism Respiration
High-frequency oscillatory ventilation (HFOV) has been proposed as gentle ventilation strategy to prevent lung injury in the preterm infant. High-frequency jet ventilation leads to dimensional and mechanical airway deformation in animal airway models, which is consistent with translational studies demonstrating the impact of oxygen and biophysical stresses on normal airway cellular function. There is an overall paucity of clinical and cellular data on the impact of HFOV on the conducting airway. We developed an innovative method to test the impact of the clinical HFO Ventilator (SensorMedics 3100A) on human epithelial cell function. In this translational model, we were able to study the differential effects of biophysical stress due to HFOV independently and in combination with hyperoxia on a direct cellular level of the conducting airway system. Additionally, we could demonstrate that hyperoxia and pressure by HFOV independently resulted in significant cell dysfunction and inflammation, while the combination of HFOV and hyperoxia had a synergistic effect, resulting in greater cell death. Traditionally, large-animal models are used to analyze the impact of clinical ventilators on lung cellular function. In our dual-chamber model, we interface high-frequency oscillatory ventilation (HFOV) directly with airway cells to study the effects of HFOV independently and combined with hyperoxia. Therefore, it is possible to study the preclinical impact of interventional factors without the high cost of animal models, thus reducing staff, time, as well as animal sparing.

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4 citations in Web of Science
4 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Physiology
Sport Sciences
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