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Journal article
A useful epitope tag derived from maltose binding protein
Protein science, v 30(6), pp 1235-1246
Jun 2021
PMID: 33896065
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Maltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross‐reactivity to other proteins in an Escherichia coli lysate. This high level of specificity suggested that MBP contains an epitope that could prove useful as a purification and visualization tag for proteins expressed in E. coli. To discover the MBP epitope, a co‐crystal structure was determined for MBP bound to its antibody and four amino acids of MBP were identified as critical for the binding interaction. Fusions of various fragments of MBP to the glutathione S‐transferase protein were engineered in order to identify the smallest fragment still recognized by the α‐MBP antibody. Stabilization of the epitope via mutational engineering resulted in a minimized 14 amino‐acid tag.
PDB Code(s): 7JTR;
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Details
- Title
- A useful epitope tag derived from maltose binding protein
- Creators
- Marine Lénon - Institut PasteurNa Ke - New England BiolabsGuoping Ren - New England BiolabsMegan E. Meuser - Drexel UniversityPatrick J. Loll - Drexel UniversityPaul Riggs - New England BiolabsMehmet Berkmen - New England Biolabs
- Publication Details
- Protein science, v 30(6), pp 1235-1246
- Publisher
- John Wiley & Sons, Inc
- Number of pages
- 12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000652472400011
- Scopus ID
- 2-s2.0-85106292946
- Other Identifier
- 991019170465704721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology