Journal article
ATP triggered drug release and DNA co-delivery systems based on ATP responsive aptamers and polyethylenimine complexes
Journal of materials chemistry. B, Materials for biology and medicine, v 4(21), pp 3832-3841
07 Jun 2016
PMID: 32263321
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Stimuli-responsive nanocarriers for anticancer drug and gene co-delivery are a promising strategy in cancer therapy due to their combination of chemotherapy and gene therapy. In this work, we developed a facile and effective method to fabricate stimuli-responsive nanocarriers for anticancer drug and gene co-delivery based on complexes of polyethylenimine (PEI) with an adenosine triphosphate (ATP) responsive aptamer duplex (ARAD). No chemical reactions or complex modifications were used in the construction processes. In this system, Doxorubicin-loaded aptamer duplex and plasmid DNA (p53) can be bound by PEI by electronic interactions to form stable complexes which effectively protect the aptamer and p53 from DNase degradation. The intercalated Dox can be released on-demand by a structural change in the aptamer duplex in an ATP-rich environment. The morphology and average size of the nanocarriers were characterized by zeta potential and transmission electron microscopy (TEM). The nanocarriers exhibit lower cell toxicity in HeLa cell lines relative to PEI. RT-PCR and Western blot analysis confirmed that p53 could be effectively delivered and expressed in HeLa cells by PEI/ARAD/p53 complexes. Moreover, the apoptosis percentage of HeLa cells treated with PEI/ARAD/Dox/p53 complex increased to 40.8%, compared to 24.7% for PEI/ARAD/Dox complex and 11.5% for PEI/ARAD/p53, respectively. The result demonstrated that the combinatorial delivery of Dox and p53 by nanocarriers could induce synergistic actions and lead to effective cancer cell apoptosis.
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Details
- Title
- ATP triggered drug release and DNA co-delivery systems based on ATP responsive aptamers and polyethylenimine complexes
- Creators
- Guan-Hai Wang - Guangdong Pharmaceutical UniversityGuo-Liang Huang - Sino-American Cancer Research Institute, Dongguan Scientific Research Center, Guangdong Medical University,Dongguan,ChinaYi Zhao - Department of Microbiology and Immunology, School of Basic Medicine, Guangdong Medical University,Dongguan,ChinaXing-Xiang Pu - Hunan Cancer HospitalTong Li - Sino-American Cancer Research Institute, Dongguan Scientific Research Center, Guangdong Medical University,Dongguan,ChinaJun-Jie Deng - Drexel UniversityJian-Tao Lin - Guangdong Pharmaceutical UniversityYuzhe Zhao - Urban Health Collaborative (2015-)
- Publication Details
- Journal of materials chemistry. B, Materials for biology and medicine, v 4(21), pp 3832-3841
- Publisher
- Royal Soc Chemistry
- Number of pages
- 10
- Grant note
- 20141158 / Traditional Chinese Medicine Bureau Foundation of Guangdong Province XB1303; XB1387 / Doctoral Research Program of Guangdong Medical College 81402563 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 4CX14119G; 4CX14118 G / Special Foundation for Young Innovation Scientists of Department of Education of Guangdong Province
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Urban Health Collaborative
- Web of Science ID
- WOS:000378102600021
- Scopus ID
- 2-s2.0-84971233973
- Other Identifier
- 991019173574704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Materials Science, Biomaterials