Journal article
Ablation of the 14-3-3gamma Protein Results in Neuronal Migration Delay and Morphological Defects in the Developing Cerebral Cortex
Developmental neurobiology (Hoboken, N.J.), v 76(6), pp 600-614
Jun 2016
PMID: 26297819
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
14-3-3 proteins are ubiquitously-expressed and multifunctional proteins. There are seven isoforms in mammals with a high level of homology, suggesting potential functional redundancy. We previously found that two of seven isoforms, 14-3-3epsilon and 14-3-3zeta, are important for brain development, in particular, radial migration of pyramidal neurons in the developing cerebral cortex. In this work, we analyzed the function of another isoform, the protein 14-3-3gamma, with respect to neuronal migration in the developing cortex. We found that in utero 14-3-3gamma-deficiency resulted in delays in neuronal migration as well as morphological defects. Migrating neurons deficient in 14-3-3gamma displayed a thicker leading process stem, and the basal ends of neurons were not able to reach the boundary between the cortical plate and the marginal zone. Consistent with the results obtained from in utero electroporation, time-lapse live imaging of brain slices revealed that the ablation of the 14-3-3gamma proteins in pyramidal neurons slowed down their migration. In addition, the 14-3-3gamma deficient neurons showed morphological abnormalities, including increased multipolar neurons with a thicker leading processes stem during migration. These results indicate that the 14-3-3gamma proteins play an important role in radial migration by regulating the morphology of migrating neurons in the cerebral cortex. The findings underscore the pathological phenotypes of brain development associated with the disruption of different 14-3-3 proteins and will advance the preclinical data regarding disorders caused by neuronal migration defects.
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Details
- Title
- Ablation of the 14-3-3gamma Protein Results in Neuronal Migration Delay and Morphological Defects in the Developing Cerebral Cortex
- Creators
- Tomoka Wachi - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19129Brett Cornell - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19129Courtney Marshall - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19129Vladimir Zhukarev - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19129Peter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19129Kazuhito Toyo-oka - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania, 19129
- Publication Details
- Developmental neurobiology (Hoboken, N.J.), v 76(6), pp 600-614
- Publisher
- Wiley; United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000374847600002
- Scopus ID
- 2-s2.0-84940707433
- Other Identifier
- 991014878165504721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Developmental Biology
- Neurosciences