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Journal article
Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF
Nature communications, v 9(1), pp 2895-14
24 Jul 2018
PMID: 30042427
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Formation and turnover of branched actin networks underlies cell migration and other essential force-driven processes. Type I nucleation-promoting factors (NPFs) such as WASP recruit actin monomers to Arp2/3 complex to stimulate nucleation. In contrast, mechanisms of type II NPFs such as Abp1 (also known as HIP55 and Drebrin-like protein) are less well understood. Here, we use single-molecule analysis to investigate yeast Abp1 effects on Arp2/3 complex, and find that Abp1 strongly enhances Arp2/3-dependent branch nucleation by stabilizing Arp2/3 on sides of mother filaments. Abp1 binds dynamically to filament sides, with sub-second lifetimes, yet associates stably with branch junctions. Further, we uncover a role for Abp1 in protecting filament junctions from GMF-induced debranching by competing with GMF for Arp2/3 binding. These data, combined with EM structures of Abp1 dimers bound to Arp2/3 complex in two different conformations, expand our mechanistic understanding of type II NPFs.
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Details
- Title
- Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF
- Creators
- Siyang Guo - Brandeis UniversityOlga S. Sokolova - Lomonosov Moscow State UniversityJohnson Chung - Brandeis UniversityShae Padrick - Drexel UniversityJeff Gelles - Brandeis UniversityBruce L. Goode - Brandeis University
- Publication Details
- Nature communications, v 9(1), pp 2895-14
- Publisher
- Springer Nature
- Number of pages
- 14
- Grant note
- 17-14-00043 / Russian Science Foundation; Russian Science Foundation (RSF) 14-14-00234 / Russian Science Foundation; Russian Science Foundation (RSF) DMR-1420382 / Brandeis NSF MRSEC R01-GM063691; R01-GM098143 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01GM098143 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000439557800001
- Scopus ID
- 2-s2.0-85050591039
- Other Identifier
- 991019168092704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology