Journal article
Absence of DICER in Monocytes and Its Regulation by HIV-1
The Journal of biological chemistry, v 285(42), pp 31930-31943
15 Oct 2010
PMID: 20584909
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
MicroRNAs (miRNAs) are a class of small RNA molecules that function to control gene expression and restrict viral replication in host cells. The production of miRNAs is believed to be dependent upon the DICER enzyme. Available evidence suggests that in T lymphocytes, HIV-1 can both suppress and co-opt the host's miRNA pathway for its own benefit. In this study, we examined the state of miRNA production in monocytes and macrophages as well as the consequences of viral infection upon the production of miRNA. Monocytes in general express low amounts of miRNA-related proteins, and DICER in particular could not be detected until after monocytes were differentiated into macrophages. In the case where HIV-1 was present prior to differentiation, the expression of DICER was suppressed. MicroRNA chip results for RNA isolated from transfected and treated cells indicated that a drop in miRNA production coincided with DICER protein suppression in macrophages. We found that the expression of DICER in monocytes is restricted by miR-106a, but HIV-1 suppressed DICER expression via the viral gene Vpr. Additionally, analysis of miRNA expression in monocytes and macrophages revealed evidence that some miRNAs can be processed by both DICER and PIWIL4. Results presented here have implications for both the pathology of viral infections in macrophages and the biogenesis of miRNAs. First, HIV-1 suppresses the expression and function of DICER in macrophages via a previously unknown mechanism. Second, the presence of miRNAs in monocytes lacking DICER indicates that some miRNAs can be generated by proteins other than DICER.
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Details
- Title
- Absence of DICER in Monocytes and Its Regulation by HIV-1
- Creators
- William Coley - George Mason UniversityRachel Van Duyne - George Mason UniversityLawrence Carpio - George Mason UniversityIrene Guendel - George Mason UniversityKylene Kehn-Hall - George Mason UniversitySebastien Chevalier - National Institutes of HealthAarthi Narayanan - George Mason UniversityTruong Luu - George Washington UniversityNorman Lee - George Washington UniversityZachary Klase - National Institutes of HealthFatah Kashanchi - George Mason University
- Publication Details
- The Journal of biological chemistry, v 285(42), pp 31930-31943
- Publisher
- Elsevier
- Number of pages
- 14
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000282683000006
- Scopus ID
- 2-s2.0-77957796766
- Other Identifier
- 991021902504304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology