Journal article
Abstract 10270: Magnetically-actuated Alginate Scaffold Enhances Infiltration of Classically Activated Host-macrophages
Circulation (New York, N.Y.), v 140(Suppl_1 Suppl 1), pp A10270-A10270
19 Nov 2019
Abstract
IntroductionArtificial tissue can restore function in failing organs. Engraftment of artificial tissue requires a coordinated interaction between the implanted material and the host innate immune response. Macrophages regulate the host-material interaction immediately following implantation, and these cells influence downstream scaffold vascularization by displaying a multi-phase response that first primes the environment and then remodels ongoing regenerative processes. We investigated a novel approach for user-controlled immunomodulation after implantation that relies on material-delivered cyclic strain. We hypothesized that cyclic strain applied during early priming phases will impact the mechanosensitive host-macrophage population by enhancing the classically activated (M1) phenotype and promoting downstream angiogenesis.Methods and ResultsWe used magnetically responsive scaffolds in combination with an alternating uniform magnetic field to deliver cyclic strain to scaffold associated cells. Cell-free scaffolds were subcutaneously implanted into male, BALB/c mice, N=5. Mice were exposed to a magnetic field (30 Gauss, 0.5Hz) during the early (day 1-3 post-implantation (p.i.)), middle (day 5-8 p.i.), or late (day 12-15 p.i.) phase of the host macrophage response or kept as unexposed controls. Magnetically actuated scaffolds at day 5-8 p.i. showed an increase in the percentage of cells expressing the macrophage marker F4/80 (67.4% ± 2.29 stimulated vs. 58.9% ± 1.05 control (p<0.05), n=5). Furthermore, we observed an increase in the percentage of macrophages expressing the M1 marker CD86 (37.5% ± 2.93 stimulated vs. 23.4% ± 2.78 control (p<0.05), n=5) by t-test. To assess scaffold vascularization at day 21 p.i., we quantified the number of perfused blood vessels on histological samples. Magnetic stimulation during the early (1.25 vessels/mm ± 0.38 (p=0.052), n=10) or middle (1.22 vessels/mm ± 0.32 (p=0.059), n=10) phase promoted angiogenesis compared to control (0.25 vessels/mm ± 0.16, n=7) using the Kruskal-Wallis test.ConclusionsWe demonstrate that user-controlled, mechanically active materials can modulate the host-macrophage response after implantation and promote angiogenesis.
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Details
- Title
- Abstract 10270: Magnetically-actuated Alginate Scaffold Enhances Infiltration of Classically Activated Host-macrophages
- Creators
- Lindsay Steele - Drexel UniversityKara Spiller - Drexel UniversitySmadar Cohen - Avram and Stella Goldstein-Goren Dept of Biotechnology Engineering, Ben-Gurion Univ of the Negev, Beer-Sheva, IsraelBoris Polyak - Drexel University
- Publication Details
- Circulation (New York, N.Y.), v 140(Suppl_1 Suppl 1), pp A10270-A10270
- Publisher
- by the American College of Cardiology Foundation and the American Heart Association, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Surgery
- Other Identifier
- 991019280040904721