Journal article
Abstract 4934: Detection of HBV-host junction DNA sequences in urine of patients with hepatocellular carcinoma
Cancer research (Chicago, Ill.), v 76(14_Supplement), pp 4934-4934
15 Jul 2016
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human urine has been shown to contain DNA from circulation. In this study, we aim to provide unambiguous evidence that urine contains hepatocellular carcinoma (HCC)-derived DNA, and thus can be used for detecting HCC-associated DNA markers. We used novel hepatitis B virus (HBV)-host junction sequences (HBV-JSs), created by viral integration in the human genome, as unique markers to track the HBV-integrated DNA from tumor tissues to their corresponding urine samples. An HBV DR1-2 enriched Next-Generation sequencing (NGS) assay was developed to identify the major HBV-JSs in 15 HBV-HCC tissues. Upon validation of the major HBV-JSs by Sanger sequencing, a short-amplicon PCR assay tailed for each HBV-JS was developed to test the junctions existence in the corresponding urine DNA. 15 major HBV-JSs were identified by the HBV DR1-2 enriched NGS assay from 15 HBV related HCC (HBV-HCC) tissues, 13 of which were validated by Sanger sequencing. By using HBV-JS specific short-amplicon junction PCR assays, we detected and confirmed six of nine HBV-JSs for which there were matching urine samples. Urine contains detectable major HBV-JSs derived from HCC, and thus can be used for liquid biopsies to study not only the complexities of HBV-JS species during chronic HBV infection and carcinogenesis, but also other HCC-related DNA modifications for the early detection of HCC and disease management.
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Details
- Title
- Abstract 4934: Detection of HBV-host junction DNA sequences in urine of patients with hepatocellular carcinoma
- Creators
- Selena Lin - Drexel UniversityEvan Trauger - Baruch S. Blumberg InstituteBenjamin Song - Baruch S. Blumberg InstituteLing Lan - Zhengzhou UniversityPatrick Jongeneel - JBS Science (United States)Emilie Thompson - Baruch S. Blumberg InstituteMalcolm Hoffman - Baruch S. Blumberg InstituteSurbhi Jain - JBS Science (United States)Ting-Tsung ChangTimothy Block - Baruch S. Blumberg InstituteWei Song - JBS Science (United States)Ying-Hsiu Su
- Publication Details
- Cancer research (Chicago, Ill.), v 76(14_Supplement), pp 4934-4934
- Publisher
- American Association for Cancer Research (AACR)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000389940601204
- Other Identifier
- 991021463688104721
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- Web of Science research areas
- Oncology