Journal article
Accurate selection of a 5' splice site requires sequences within fibronectin alternative exon B
Nucleic acids research, v 27(19), pp 3945-3952
01 Oct 1999
PMID: 10481035
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Inclusion of fibronectin alternative exon B in mRNA is developmentally regulated. Here we demonstrate that exon B contains two unique purine-rich sequence tracts, PRE1 and PRE2, that are important for proper 5' splice site selection both in vivo and in vitro. Targeted mutations of both PREs decreased the inclusion of exon B in the mRNA by 50% in vivo. Deletion or mutation of the PREs reduced removal of the downstream intron, but not the upstream intron, and induced the activation of cryptic 5' splice sites in vitro. PRE-mediated 5' splice selection activity appears sensitive to position and sequence context. A well characterized exon sequence enhancer that normally acts on the upstream 3' splice site can partially rescue proper exon B 5' splice site selection. In addition, we found that PRE 5' splice selection activity was preserved when exon B was inserted into a heterologous pre-mRNA substrate. Possible roles of these unique activities in modulating exon B splicing are considered.
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Details
- Title
- Accurate selection of a 5' splice site requires sequences within fibronectin alternative exon B
- Creators
- B A Kuo - Department of Medicine, Division of Gastroenterology and Hepatology, Thomas Jefferson University, Philadelphia, PA 19107, USAP A Norton
- Publication Details
- Nucleic acids research, v 27(19), pp 3945-3952
- Publisher
- Oxford University Press; England
- Grant note
- R01-GM47453 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000082980600021
- Scopus ID
- 2-s2.0-0033214806
- Other Identifier
- 991014878342804721
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- Web of Science research areas
- Biochemistry & Molecular Biology