Journal article
Acetylcholine receptor gating is influenced by the polarity of amino acids at position 9' in the M2 domain
The Journal of membrane biology, v 174(3), pp 191-197
01 Apr 2000
PMID: 10758172
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Abstract
Ligand-gated ion channels contain a conserved leucine at position 9' (L9') in the M2 transmembrane domain. We used multiple substitutions at this position in the gamma subunit of the mouse acetylcholine receptor (AChR) (gammaL9') to examine the role of residue polarity at this position in the gating process at both the macroscopic and single-channel levels. The midpoint of the macroscopic dose-response relationship (EC(50)) and the channel closing rate constant, alpha, decreased as the polarity of the residue at that position increased, suggesting a stabilization of the open state of the channel. Both parameters showed similar dependencies on the polarity of the substituted residue. These data support the notion that during AChR gating, the amino acid at the 9' position moves into a polar environment, and that interactions between this residue and the polar environment determine the stability of the open state. Since this residue is conserved in all other members of the ligand-gated ion channel family, we suggest that a similar mechanism applies to the other members of the family.
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Details
- Title
- Acetylcholine receptor gating is influenced by the polarity of amino acids at position 9' in the M2 domain
- Creators
- A V Kosolapov - Department of Pharmacology and Physiology, MCP Hahnemann University, Philadelphia, PA 19129, USAG N FilatovM M White
- Publication Details
- The Journal of membrane biology, v 174(3), pp 191-197
- Publisher
- Springer Nature; United States
- Grant note
- NS 23885 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000086395600002
- Scopus ID
- 2-s2.0-0034176414
- Other Identifier
- 991014878096904721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- Physiology