Journal article
Activated CaMKII Couples GluN2B and Casein Kinase 2 to Control Synaptic NMDA Receptors
Cell reports (Cambridge), v 3(3), pp 607-614
01 Mar 2013
PMID: 23478024
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Synaptic activity triggers a profound reorganization of the molecular composition of excitatory synapses. For example, NMDA receptors are removed from synapses in an activity- and calcium-dependent manner, via casein kinase 2 (CK2) phosphorylation of the PDZ ligand of the GluN2B subunit (S1480). However, how synaptic activity drives this process remains unclear because CK2 is a constitutively active kinase, which is not directly regulated by calcium. We show here that activated CaMKII couples GluN2B and CK2 to form a trimolecular complex and increases CK2-mediated phosphorylation of GluN2B S1480. In addition, a GluN2B mutant, which contains an insert to mimic the GluN2A sequence and cannot bind to CaMKII, displays reduced S1480 phosphorylation and increased surface expression. We find that although disrupting GluN2B/CaMKII binding reduces synapse number, it increases synaptic-GluN2B content. Therefore, the GluN2B/CaMKII association controls synapse density and PSD composition in an activity-dependent manner, including recruitment of CK2 for the removal of GluN2B from synapses.
Metrics
Details
- Title
- Activated CaMKII Couples GluN2B and Casein Kinase 2 to Control Synaptic NMDA Receptors
- Creators
- Antonio Sanz-Clemente - National Institute of Neurological Disorders and StrokeJohn A. Gray - Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94143 USAKyle A. Ogilvie - National Institute of Neurological Disorders and StrokeRoger A. Nicoll - University of California, San FranciscoKatherine W. Roche - National Institute of Neurological Disorders and Stroke
- Publication Details
- Cell reports (Cambridge), v 3(3), pp 607-614
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- NINDS Intramural Research Program; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) K08MH100562 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) NIMH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) NARSAD Hammerschlag Family Investigator; NARSAD R00AG041225 / NATIONAL INSTITUTE ON AGING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) ZIANS002994 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) NARSAD Young Investigator Award; NARSAD
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000321896000003
- Scopus ID
- 2-s2.0-84875808267
- Other Identifier
- 991020100084804721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology