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Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells
Journal article   Open access   Peer reviewed

Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells

Katrin Roedder, Juliane Moritz, Vandana Miller, Klaus-Dieter Weltmann, Hans-Robert Metelmann, Rajesh Gandhirajan and Sander Bekeschus
Applied sciences, v 9(4), p660
15 Feb 2019
DOI: https://doi.org/10.3390/app9040660
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.3390/app9040660View
Published, Version of Record (VoR) Open

Abstract

Chemistry Chemistry, Multidisciplinary Engineering Engineering, Multidisciplinary Materials Science Materials Science, Multidisciplinary Physical Sciences Physics Physics, Applied Science & Technology Technology
Recent advances in melanoma therapy increased median survival in patients. However, death rates are still high, motivating the need of novel avenues in melanoma treatment. Cold physical plasma expels a cocktail of reactive species that have been suggested for cancer treatment. High species concentrations can be used to exploit apoptotic redox signaling pathways in tumor cells. Moreover, an immune-stimulatory role of plasma treatment, as well as plasma-killed tumor cells, was recently proposed, but studies using primary immune cells are scarce. To this end, we investigated the role of plasma-treated murine B16F10 melanoma cells in modulating murine immune cells' activation and marker profile. Melanoma cells exposed to plasma showed reduced metabolic and migratory activity, and an increased release of danger signals (ATP, CXCL1). This led to an altered cytokine profile with interleukin-1 (IL-1) and CCL4 being significantly increased in plasma-treated mono- and co-cultures with immune cells. In T cells, plasma-treated melanoma cells induced extracellular signal-regulated Kinase (ERK) phosphorylation and increased CD28 expression, suggesting their activation. In monocytes, CD115 expression was elevated as a marker for activation. In summary, here we provide proof of concept that plasma-killed tumor cells are recognized immunologically, and that plasma exerts stimulating effects on immune cells alone.

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31 citations in Web of Science
30 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#3 Good Health and Well-Being

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Chemistry, Multidisciplinary
Engineering, Multidisciplinary
Materials Science, Multidisciplinary
Physics, Applied
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