Journal article
Activation of groups of excitatory neurons in the mammalian spinal cord or hindbrain evokes locomotion
Nature neuroscience, v 13(2), pp 246-U143
01 Feb 2010
PMID: 20081850
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Central pattern generators (CPGs) are spinal neuronal networks required for locomotion. Glutamatergic neurons have been implicated as being important for intrinsic rhythm generation in the CPG and for the command signal for initiating locomotion, although this has not been demonstrated directly. We used a newly generated vesicular glutamate transporter 2-channelrhodopsin2-yellow fluorescent protein (Vglut2-ChR2-YFP) mouse to directly examine the functional role of glutamatergic neurons in rhythm generation and initiation of locomotion. This mouse line expressed ChR2-YFP in the spinal cord and hindbrain. ChR2-YFP was reliably expressed in Vglut2-positive cells and YFP-expressing cells could be activated by light. Photo-stimulation of either the lumbar spinal cord or the caudal hindbrain was sufficient to both initiate and maintain locomotor-like activity. Our results indicate that glutamatergic neurons in the spinal cord are critical for initiating or maintaining the rhythm and that activation of hindbrain areas containing the locomotor command regions is sufficient to directly activate the spinal locomotor network.
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Details
- Title
- Activation of groups of excitatory neurons in the mammalian spinal cord or hindbrain evokes locomotion
- Creators
- Martin Hagglund - Karolinska InstitutetLotta Borgius - Karolinska InstitutetKimberly J. Dougherty - Karolinska InstitutetOle Kiehn - Karolinska Institutet
- Publication Details
- Nature neuroscience, v 13(2), pp 246-U143
- Publisher
- Springer Nature
- Number of pages
- 8
- Grant note
- Karolinska Institutet's foundation 0701166 / National Science Foundation; National Science Foundation (NSF) Swedish Medical Research Council; Swedish Medical Research Council (SMRC) R01NS040795-08 / US National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA European Union; European Commission Friends of Karolinska Intitutet Soderbergs Foundation R01NS040795 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000273985800021
- Scopus ID
- 2-s2.0-75549086090
- Other Identifier
- 991020100204004721
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- Web of Science research areas
- Neurosciences