Journal article
Activation of phospholipase D is not mediated by direct phosphorylation on tyrosine residues
Biochimica et biophysica acta. Molecular and cell biology of lipids, v 1631(3)
2003
PMID: 12668176
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The activation of phospholipase D (PLD) in PC12/PC2 pheochromocytoma cells involves a tyrosine kinase. However, it is not clear whether this is due to direct phosphorylation of the enzyme or some other intermediary protein. In this manuscript, we examined this issue by two methods: (1) immunoprecipitation of phosphotyrosine containing proteins and assay of phospholipase D; (2) overexpression of HA–phospholipase D2 and susbsequent immunoprecipitation. The only agent that caused phosphorylation of phospholipase D on tyrosine residues was the phosphatase inhibitor, peroxyvanadate. Other agents that activate phospholipase D, including bradykinin, ionomycin, and phorbol dibutyrate did not cause phosphorylation of the enzyme. In addition, there was a lack of correlation between the peroxyvanadate-mediated phosphorylation and activation of phospholipase D, both in terms of time course and concentration dependence. These data demonstrate that phospholipase D is directly phosphorylated on tyrosine residues. However, phosphorylation of tyrosine residues does not correlate with activation of the enzyme.
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Details
- Title
- Activation of phospholipase D is not mediated by direct phosphorylation on tyrosine residues
- Creators
- Sanjoy MehtaJeff MaglioMike S KobayashiAndrea M SippleJoel Horwitz
- Publication Details
- Biochimica et biophysica acta. Molecular and cell biology of lipids, v 1631(3)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000182206100005
- Scopus ID
- 2-s2.0-0037426321
- Other Identifier
- 991014878006104721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology