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Journal article
Acute Adipocyte Viability After Third-Generation Ultrasound-Assisted Liposuction
Aesthetic surgery journal, v 33(5), pp 698-704
01 Jul 2013
PMID: 23718978
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background: Although clinical evidence of successful autologous fat transfer (AFT) using third-generation ultrasound-assisted liposuction (UAL) is readily available, no study has quantified adipocyte viability using standardized methods.
Objectives: The authors assess acute adipocyte viability following fat aspiration as a first step in determining the overall efficacy of using third-generation UAL for AFT.
Methods: Lipoaspirate samples were collected from patients who underwent elective liposuction procedures at multiple surgery centers. Patients with a history of bleeding disorders, diabetes, human immunodeficiency virus, or lipoatrophy disorders were excluded. The UAL system (VASER; Sound Surgical Technologies, Inc, Louisville, Colorado) was set at 60% amplitude in pulsed mode with vacuum aspiration of 15 in Hg or less. Laboratory analysis included free lipid volume, viability via lipolysis and propidium iodide staining, and cytological analysis, including cell surface protein examination and hematoxylin and eosin staining.
Results: The lipolysis assay revealed metabolically active adipocytes with a mean (SD) correlative viability of 85.1% (11%). Direct measures of acute viability via propidium iodide staining resulted in a mean (SD) viability measure of 88.7% (3.5%). Both mean values are within the historical range reported from syringe and vacuum-assisted lipoaspiration. Aqueous and lipid contents were favorably reduced after washing and filtering (Puregraft system; Cytori Therapeutics, Inc, San Diego, California). Cellular phenotypes identified were primarily white blood cells or vascular endothelial and vascular associated cells.
Conclusions: Adipose tissue acquired via third-generation UAL is viable at harvest and is potentially a suitable source for autologous fat grafts. These results confirm reported clinical successes utilizing third-generation ultrasound lipoaspirate for AFT.
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Details
- Title
- Acute Adipocyte Viability After Third-Generation Ultrasound-Assisted Liposuction
- Creators
- Mark E. Schafer (Corresponding Author) - Sound Surgical Technologies (United States, Louisville)Kevin C. Hicok - Cytori Therapeutics (United States)Daniel C. Mills - Loma Linda UniversitySteve R. Cohen - University of California San DiegoJames J. Chao - University of California San Diego
- Publication Details
- Aesthetic surgery journal, v 33(5), pp 698-704
- Publisher
- Oxford Univ Press
- Number of pages
- 7
- Grant note
- Sound Surgical Technologies, LLC (Louisville, Colorado)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000323030700010
- Scopus ID
- 2-s2.0-84879721408
- Other Identifier
- 991019176638204721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Surgery