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Journal article
Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
Cell reports (Cambridge), v 34(3), pp 108640-108640
19 Jan 2021
PMID: 33472084
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In multicellular eukaryotes, RNA polymerase (Pol) II pauses transcription ~30-50 bp after initiation. While the budding yeast Saccharomyces has its transcription mechanisms mostly conserved with other eukaryotes, it appears to lack this fundamental promoter-proximal pausing. However, we now report that nearly all yeast genes, including constitutive and inducible genes, manifest two distinct transcriptional stall sites that are brought on by acute environmental signaling (e.g., peroxide stress). Pol II first stalls at the pre-initiation stage before promoter clearance, but after DNA melting and factor acquisition, and may involve inhibited dephosphorylation. The second stall occurs at the +2 nucleosome. It acquires most, but not all, elongation factor interactions. Its regulation may include Bur1/Spt4/5. Our results suggest that a double Pol II stall is a mechanism to downregulate essentially all genes in concert.
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Details
- Title
- Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
- Creators
- Nitika Badjatia - Pennsylvania State UniversityMatthew J Rossi - Pennsylvania State UniversityAlain R Bataille - Pennsylvania State UniversityChitvan Mittal - Pennsylvania State UniversityWilliam K M Lai - Pennsylvania State UniversityB Franklin Pugh - Pennsylvania State University
- Publication Details
- Cell reports (Cambridge), v 34(3), pp 108640-108640
- Publisher
- Elsevier
- Grant note
- R01 GM059055 / NIGMS NIH HHS R01 HG004160 / NHGRI NIH HHS R01 ES013768 / NIEHS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000609627300010
- Scopus ID
- 2-s2.0-85099607759
- Other Identifier
- 991019168207704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology