Journal article
Addition of glucosamine and mannose to nascent immunoglobulin heavy chains
Biochemistry (Easton), v 16(20), pp 4490-4497
01 Oct 1977
PMID: 410438
Abstract
We have investigated the process of protein glycosylation in an attempt to answer the question of whether glucosamine and mannose are added to nascent chains prior to chain completion or only to completed chains after release from the ribosome. The MPC 11 mouse plasmacytoma cell line used in these studies synthesizes a glycosylated gamma2b heavy chain which accounts for 12% of the total protein synthesis. Nascent chains were separated from completed chains by ion-exchange chromatography of solubilized ribosomes on QAE-Sephadex. Our results indicate that both glucosamine and mannose are incorporated into nascent heavy chains prior to chain completion and release from the ribosome. Gel analysis of specifically immunoprecipitated nascent chains indicates that the carbohydrate moiety can be added to the nascent heavy chains very soon after the presumptive asparaginyl glycosylation site (CH2 domain) is synthesized on the ribosome.
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Details
- Title
- Addition of glucosamine and mannose to nascent immunoglobulin heavy chains
- Creators
- L W BergmanW M Kuehl
- Publication Details
- Biochemistry (Easton), v 16(20), pp 4490-4497
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1977DW50400025
- Scopus ID
- 2-s2.0-0017706873
- Other Identifier
- 991020547447404721