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Age-Related Decrease in Omega Conotoxin Binding to Rat Cardiac Synaptosomes
Journal article   Peer reviewed

Age-Related Decrease in Omega Conotoxin Binding to Rat Cardiac Synaptosomes

Ajay M. Parikh, Mark D. Johnson, Vince Aloyo, H. Beal McIlvain, Vinay Bhavsar, Jay Roberts and James R Roberts
Proceedings of the Society for Experimental Biology and Medicine, v 199(4), pp 453-458
Apr 1992
DOI: https://doi.org/10.3181/00379727-199-43380
PMID: 1549624
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Abstract A cardiac synaptosomal preparation developed by this laboratory was used to study neuronal calcium channels in aging rat heart. Ca2+ channels were quantified by measuring binding of iodinated omega conotoxin, which is reported to specifically block neuronal Ca2+ channels. We determined the binding of [125I]-omega conotoxin GVIA to a synaptosomal preparation from the hearts of 6- and 24-month-old male Fisher 344 rats. The maximum number of binding sites (Bmax ± SD, fmol/mg protein) is lower in preparations from 24-month (2.2 ± 0.6) than from 6-month (3.4 ± 0.7)-old rats. This decrease in number of binding sites suggests an age-related reduction in the number of neuronal calcium channels. Since calcium is essential for exocytotic release of norepinephrine and is made available intracellularly through neuronal calcium channels, the reduction in neuronal calcium channel number may explain, in part, our previous observations of diminished release of norepinephrine in senescent hearts. [P.S.E.B.M. 1992, Vol 199]

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Web of Science research areas
Medicine, Research & Experimental
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