Journal article
Age-related decline in beta adrenergic and adenosine A1 receptor function in the heart are attenuated by dietary restriction
The Journal of pharmacology and experimental therapeutics, v 285(1)
Apr 1998
PMID: 9536009
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Previously published reports from this laboratory have shown that the antiadrenergic effect of adenosine A1 agonists declines with age in the rat heart [ J Mol Cell Cardiol 29:593-602] and that this decline may be caused by a decrease in coupling between adenosine A1 receptors (AdoA1R) and guanine nucleotide-binding proteins [ Circ Res 81:1065-1071]. Dietary restriction (DR; 60% calories of ad libitum) has been shown to attenuate age-related changes in cellular signal transduction pathways. Therefore, the present study investigated whether DR altered the age-related changes in AdoA1R-mediated function in senescent rat hearts. Ventricular membranes were isolated from the hearts of ad libitum (AL) fed and DR male F344 rats that were 6, 12 and 24 months of age. In AL rats, there was an age-related decline in isoproterenol (ISO)-stimulated adenylyl cyclase when compared with the 6-month-old rats. The decline in ISO-stimulated cyclase was attenuated in DR animals. In AL rats, inhibition of ISO-stimulated adenylyl cyclase by the AdoA1R agonist, N6-p-sulfophenyladenosine (SPA) decreased with age. In DR rats, the age-related decline in inhibition was attenuated. Previous results from this laboratory indicated that in AL fed rats, there was an age-related decrease in the percentage of high-affinity binding sites for SPA, from 55% at 6 months to 23% at 24 months. Diet restriction attenuated this age-related shift in high-affinity binding sites so that the percentage of high-affinity sites at 24 months was 42%. Our results suggest that DR maintains AdoA1R function by preventing a loss of high-affinity AdoA1R sites.
Metrics
5 Record Views
Details
- Title
- Age-related decline in beta adrenergic and adenosine A1 receptor function in the heart are attenuated by dietary restriction
- Creators
- E Gao - Department of Pharmacology, MCP Hahnemann School of Medicine, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19129, USAD L SnyderJ RobertsE FriedmanG CaiA PellegJ Horwitz
- Publication Details
- The Journal of pharmacology and experimental therapeutics, v 285(1)
- Publisher
- United States
- Grant note
- KO7 AG 00532 / NIA NIH HHS AG 11060 / NIA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000072972200023
- Scopus ID
- 2-s2.0-0031956231
- Other Identifier
- 991014877961404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Pharmacology & Pharmacy